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Directly watching biomolecules in action by high-speed atomic force microscopy
https://doi.org/10.24517/00049633
https://doi.org/10.24517/00049633d3e30c3b-3551-49d7-9567-ac0ace15f05c
| 名前 / ファイル | ライセンス | アクション |
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SC-PR-ANDO-T-421.pdf (1.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2017-12-28 | |||||||
| タイトル | ||||||||
| タイトル | Directly watching biomolecules in action by high-speed atomic force microscopy | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00049633 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Ando, Toshio
× Ando, Toshio |
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| 著者別表示 |
安藤, 敏夫
× 安藤, 敏夫
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| 提供者所属 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 金沢大学理工研究域バイオＡＦＭ先端研究センター | |||||||
| 書誌情報 |
Biophysical Reviews 巻 9, 号 4, p. 421-429, 発行日 2017-08-01 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1867-2450 | |||||||
| DOI | ||||||||
| 関連タイプ | isVersionOf | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1007/s12551-017-0281-7 | |||||||
| 出版者 | ||||||||
| 出版者 | Springer Verlag | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Proteins are dynamic in nature and work at the single molecule level. Therefore, directly watching protein molecules in dynamic action at high spatiotemporal resolution must be the most straightforward approach to understanding how they function. To make this observation possible, high-speed atomic force microscopy (HS-AFM) has been developed. Its current performance allows us to film biological molecules at 10–16 frames/s, without disturbing their function. In fact, dynamic structures and processes of various proteins have been successfully visualized, including bacteriorhodopsin responding to light, myosin V walking on actin filaments, and even intrinsically disordered proteins undergoing order/disorder transitions. The molecular movies have provided insights that could not have been reached in other ways. Moreover, the cantilever tip can be used to manipulate molecules during successive imaging. This capability allows us to observe changes in molecules resulting from dissection or perturbation. This mode of imaging has been successfully applied to myosin V, peroxiredoxin and doublet microtubules, leading to new discoveries. Since HS-AFM can be combined with other techniques, such as super-resolution optical microscopy and optical tweezers, the usefulness of HS-AFM will be further expanded in the near future. © 2017, International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany. | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Embargo Period 12 months | |||||||
| 権利 | ||||||||
| 権利情報 | Copyright © Springer Verlag | |||||||
| 権利URI | ||||||||
| 権利情報 | The final publication is available at www.springerlink.com/article/10.1007/s12551-017-0281-7 | The final publication is available at www.springerlink.com/article/10.1007/s12551-017-0281-7 | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
