| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-12-28 |
| タイトル |
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タイトル |
Nanovisualization of proteins in action using high-speed AFM |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.24517/00049634 |
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ID登録タイプ |
JaLC |
| 著者 |
Uchihashi, Takayuki
Kodera, Noriyuki
Ando, Toshio
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| 著者別表示 |
内橋, 貴之
古寺, 哲幸
安藤, 敏夫
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| 提供者所属 |
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内容記述タイプ |
Other |
|
内容記述 |
金沢大学理工研究域バイオＡＦＭ先端研究センター |
| 書誌情報 |
Single-molecule Studies of Proteins
p. 119-147,
発行日 2013-01-01
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| ISBN |
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識別子タイプ |
ISBN |
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関連識別子 |
978-146144921-8 |
| ISBN |
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識別子タイプ |
ISBN |
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関連識別子 |
978-146144920-1 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1007/978-1-4614-4921-8_5 |
| 出版者 |
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出版者 |
Springer New York |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The dynamics of proteins, such as their conformational change and dynamic interplay with interaction partners, is crucial to their biological functions. To reveal the dynamic behavior of proteins, single-molecule approaches are indispensable because the molecules behave in an unsynchronized manner, which makes it difficult to monitor their dynamics by ensemble average experiments. Fluorescence microscopy has been widely used to study the functional behavior of single-protein molecules (Peterman et al. 2004; Joo et al. 2008; Roy et al. 2008; Yanagida and Ishii 2008). However, it only visualizes featureless fluorescent spots, meaning that protein molecules themselves are invisible. Moreover, what we can analyze using fluorescence techniques is limited to the behavior of a selected portion where a fluorophore is attached. Therefore, inferences have to be made to bridge the gap between the observed behavior of fluorescent spots and the actual behavior of labeled protein molecules. Consequently, it takes a considerably long time to reach a persuasive conclusion on how a protein dynamically behaves to function. © Springer Science+Business Media New York 2013. |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Embargo Period 12 months |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Book Chapter |
| 権利 |
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権利情報 |
Copyright © Springer New York |
| 権利URI |
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権利情報 |
The final publication is available at www.springerlink.com/article/10.1007/978-1-4614-4921-8_5 | The final publication is available at www.springerlink.com/article/10.1007/978-1-4614-4921-8_5 |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
SC-PR-ANDO-T-119.pdf (4.5 MB)
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