| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2018-01-25 |
| タイトル |
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タイトル |
Tumor-derived trypsin enhances proliferation of intrahepatic cholangiocarcinoma cells by activating protease-activated receptor-2 |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.24517/00049837 |
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ID登録タイプ |
JaLC |
| 著者 |
Nakanuma, Shinichi
Tajima, Hidehiro
Okamoto, Koichi
Hayashi, Hironori
Nakagawara, Hisatoshi
Onishi, Ichiro
Takamura, Hiroyuki
Kitagawa, Hirohisa
Fushida, Sachio
Tani, Takashi
Fujimura, Takashi
Kayahara, Masato
Ohta, Tetsuo
Wakayama, Tomohiko
Iseki, Shoichi
Harada, Shinichi
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| 著者別表示 |
中村, 信一
田島, 秀浩
岡本, 浩一
林, 泰寛
中川原, 寿俊
高村, 博之
北川, 裕久
伏田, 幸夫
谷, 卓
藤村, 隆
萱原, 正都
太田, 哲生
若山, 友彦
井関, 尚一
原田, 真市
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| 提供者所属 |
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内容記述タイプ |
Other |
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内容記述 |
金沢大学医薬保健研究域医学系 |
| 書誌情報 |
International Journal of Oncology
巻 36,
号 4,
p. 793-800,
発行日 2010-04
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1019-6439 |
| item_4_source_id_11 |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10992511 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.3892/ijo-00000555 |
| 出版者 |
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出版者 |
Spandidos Publications |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
In primary malignant liver tumors, trypsinogen-immunoreactivity was present in 70% of intrahepatic cholangiocarcinoma (ICC) specimens, but absent in hepato-cellular carcinoma (HCC) specimens. We suggest the secretion of trypsinogen to be a key difference in biological behavior between ICC and HCC cells. The purpose of this study was to investigate the secretion of tumor-derived trypsin and the expression of its specific receptor, protease-activated receptor-2 (PAR-2), in ICC using cell lines and surgical specimens. The expression of trypsinogen-1 mRNA was observed in three of four ICC cell lines, but none of three HCC cell lines. Western blot analysis detected trypsinogen-1 in serum-free conditioned medium from one of the ICC cell lines positive for the mRNA. Gelatin zymography revealed a gelatinolytic activity for trypsin, the activated form of trypsinogen, in the same conditioned medium. PAR-2 mRNA and protein were observed in ICC cell lines. The proliferative activity of ICC cells was increased by concentrations of trypsin as low as 10 nM, and peaked at 100 nM. The effect of trypsin was suppressed by a serine protease inhibitor, gabexate mesilate. PAR-2 expression was detected in 64% of ICC surgical specimens immunohistochemically. In addition, stroma fibroblasts expressed PAR-2 in 52% of ICC specimens. These results suggest that trypsinogen-1 contributes to the growth of ICC cells and also tumor-associated fibroblasts. |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Embargo Period 6 months |
| 権利 |
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権利情報 |
Copyright © Spandidos Publications |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
ME-PR-ISEKI-S-793.pdf (770.6 kB)
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