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Laser microdissection of cellular compartments for expression analyses in cancer models
https://doi.org/10.24517/00050479
https://doi.org/10.24517/00050479fc53833f-1628-4432-b77a-136adb1bef3a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
FR-PR-OSHIMA-M-143.pdf (935.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2018-04-06 | |||||||
| タイトル | ||||||||
| タイトル | Laser microdissection of cellular compartments for expression analyses in cancer models | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00050479 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Han, Tae-Su
× Han, Tae-Su× Oshima, Masanobu |
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| 著者別表示 |
大島, 正伸
× 大島, 正伸
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| 提供者所属 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 金沢大学新学術創成研究機構ナノ生命科学研究所 | |||||||
| 書誌情報 |
Methods in Molecular Biology 巻 1725, p. 143-153, 発行日 2018-01-01 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1064-3745 | |||||||
| NCID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA11067778 | |||||||
| DOI | ||||||||
| 関連タイプ | isVersionOf | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1007/978-1-4939-7568-6_12 | |||||||
| 出版者 | ||||||||
| 出版者 | Humana Press Inc. / Springer | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Cancer tissues are composed of various cell types including cancer cells, cancer-associated fibroblasts (CAFs), tumor-associated macrophages (TAMs) and endothelial cells. These surrounding stromal cells form the tumor microenvironment, partly through the inflammatory response, which plays an important role in the development and malignant progression of cancer. It is therefore important to examine the expression profiles and protein modifications of each cellular component independently to decipher the interaction between the tumor cells and the microenvironment. We herein describe a protocol for laser microdissection, which allows for the individual cellular compartments to be collected separately. This will allow us to perform real-time RT-PCRs and microarray analyses of specific cell types in tumor tissues. © 2018, Springer Science+Business Media, LLC. | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Embargo Period 12 months | |||||||
| 権利 | ||||||||
| 権利情報 | The final publication is available at www.springerlink.com/article/10.1007/978-1-4939-7568-6_12 | |||||||
| 権利 | ||||||||
| 権利情報 | Copyright © 2018, Springer Science+Business Media, LLC. | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
