| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-03-08 |
| タイトル |
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タイトル |
Liquid-based urine cytology as a tool for detection of human papillomavirus, Mycoplasma spp., and Ureaplasma spp. in men |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.24517/00053648 |
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ID登録タイプ |
JaLC |
| 著者 |
Kawaguchi, Shohei
Shigehara, Kazuyoshi
Sasagawa, Toshiyuki
Shimamura, Masayoshi
Nakashima, Takao
Sugimoto, Kazuhiro
Nakashima, Kazufumi
Furubayashi, Keiichi
Namiki, Mikio
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| 著者別表示 |
川口, 昌平
重原, 一慶
笹川, 寿之
島村, 正喜
中嶋, 孝夫
杉本, 和宏
中嶋, 一史
古林, 敬一
並木, 幹夫
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| 提供者所属 |
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内容記述タイプ |
Other |
|
内容記述 |
金沢大学医薬保健研究域医学系 |
| 書誌情報 |
Journal of Clinical Microbiology
巻 50,
号 2,
p. 401-406,
発行日 2012
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0095-1137 |
| NCID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00695531 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1128/JCM.05219-11 |
| 出版者 |
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出版者 |
American Society for Microbiology |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Liquid-based urine cytology (LB-URC) was evaluated for cytological diagnosis and detection of human papillomavirus (HPV), Mycoplasma, and Ureaplasma. Midstream urine samples were collected from 141 male patients with urethritis and 154 controls without urethritis, and sediment cells were preserved in liquid-based cytology solution. Urethral swabs from urethritis patients were tested for the presence of Neisseria gonorrhoeae and Chlamydia trachomatis. Papanicolaou tests were performed for cytological evaluation. HPV, Mycoplasma, and Ureaplasma genomes were determined by PCR-based methods, and localization of HPV DNA in urothelial cells was examined by in situ hybridization (ISH). The β-globin gene was positive in 97.9% of LB-URC samples from urethritis patients and in 97.4% of control samples, suggesting that high-quality cellular DNA was obtained from the LB-URC samples. HPV DNA was detected in 29 (21.0%) urethritis cases and in five (3.3%) controls (P<0.05). HPV type 16 (HPV 16) was most commonly found in urethritis patients. Cytological evaluations could be performed for 92.1% of urethritis patients and 64.3% of controls. Morphological changes suggestive of HPV infection were seen in 20.7% of the HPV-positive samples, and ISH demonstrated the presence of HPV DNA in both squamous and urothelial cells in HPV-positive samples. Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum were detected in 14.5%, 10.9%, 6.5%, and 12.3% of urethritis patients, respectively. The prevalence rates of these microorganisms (except Ureaplasma parvum) were significantly higher in urethritis cases than controls (P<0.05). LB-URC is applicable for detection of HPV, Mycoplasma, and Ureaplasma. HP infection occurs in urothelial cells, especially in gonococcal urethritis. Copyright © 2012, American Society for Microbiology. All Rights Reserved. |
| 権利 |
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|
権利情報 |
Copyright © 2012, American Society for Microbiology |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
ME-PR-NAMIKI-M-401.pdf (437.4 kB)
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