| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-04-25 |
| タイトル |
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タイトル |
MTMR4, a phosphoinositide-specific 3'-phosphatase, regulates TFEB activity and the endocytic and autophagic pathways. |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.24517/00053882 |
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ID登録タイプ |
JaLC |
| 著者 |
Pham, Hoa Q.
Yoshioka, Kazuaki
Mohri, Hiromi
Nakata, Hiroki
Aki, Sho
Ishimaru, Kazuhiro
Takuwa, Noriko
Takuwa, Yoh
|
| 著者別表示 |
吉岡, 和晃
仲田, 浩規
安藝, 翔
多久和, 典子
多久和, 陽
|
| 提供者所属 |
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内容記述タイプ |
Other |
|
内容記述 |
金沢大学医薬保健研究域医学系 |
| 書誌情報 |
Genes Cells
巻 23,
号 8,
p. 670-687,
発行日 2018-08
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1356-9597 |
| NCID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11078945 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1111/gtc.12609 |
| 出版者 |
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出版者 |
John Wiley & Sons |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Phosphatidylinositol 3-phosphate (PI(3)P) is the predominant phosphoinositide species in early endosomes and autophagosomes, in which PI(3)P dictates traffic of these organelles. Phosphoinositide levels are tightly regulated by lipid-kinases and -phosphatases; however, a phosphatase that converts PI(3)P back to phosphatidylinositol in the endosomal and autophagosomal compartments is not fully understood. We investigated the subcellular distribution and functions of myotubularin-related protein-4 (MTMR4), which is distinct among other MTMRs in that it possesses a PI(3)P-binding FYVE domain, in lung alveolar epithelium-derived A549 cells. MTMR4 was localized mainly in late endosomes and autophagosomes. MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P-enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P-enriched early and late endosomes. In amino acid- and serum-starved cells, MTMR4 knockdown decreased both autophagosomes and autolysosomes and markedly increased PI(3)P-containing autophagosomes and late endosomes, suggesting that the fusion with lysosomes of autophagosomes and late endosomes might be impaired. Notably, MTMR4 knockdown inhibited the nuclear translocation of starvation stress responsive transcription factor-EB (TFEB) with reduced expression of lysosome-related genes in starved cells. These findings indicate that MTMR4 is essential for the integrity of endocytic and autophagic pathways. © 2018 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd. |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Embargo Period 12 months |
| 権利 |
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権利情報 |
copyright © 2018 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd. |
| 著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連URI |
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識別子タイプ |
URI |
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関連識別子 |
https://onlinelibrary.wiley.com/doi/full/10.1111/gtc.12609 |
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関連名称 |
https://onlinelibrary.wiley.com/doi/full/10.1111/gtc.12609 |
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