WEKO3
アイテム
High-speed AFM reveals accelerated binding of agitoxin-2 to a K+ channel by induced fit
https://doi.org/10.24517/00054798
https://doi.org/10.24517/000547987156656d-135f-4eb0-bfee-563179d3a15d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
FR-PR-SUMIKAMA-T-0495.pdf (988.6 kB)
|
.png)
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2019-07-05 | |||||||||
| タイトル | ||||||||||
| タイトル | High-speed AFM reveals accelerated binding of agitoxin-2 to a K+ channel by induced fit | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.24517/00054798 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| 著者 |
Sumino, Ayumi
× Sumino, Ayumi× Sumikama, Takashi× Uchihashi, T.× Oki, S. |
|||||||||
| 著者別表示 |
角野, 歩
× 角野, 歩
× 炭竈, 享司
|
|||||||||
| 提供者所属 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 金沢大学ナノ生命科学研究所 | |||||||||
| 書誌情報 |
Science Advances 巻 5, 号 7, p. eaax0495, 発行日 2019-07-03 |
|||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 2375-2548 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1126/sciadv.aax0495 | |||||||||
| 出版者 | ||||||||||
| 出版者 | American Association for the Advancement of Science | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Agitoxin-2 (AgTx2) from scorpion venom is a potent blocker of K+ channels. The docking model has been elucidated, but it remains unclear whether binding dynamics are described by a two-state model (AgTx2-bound and AgTx2-unbound) or a more complicated mechanism, such as induced fit or conformational selection. Here, we observed the binding dynamics of AgTx2 to the KcsA channel using high-speed atomic force microscopy. From images of repeated binding and dissociation of AgTx2 to the channel, single-molecule kinetic analyses revealed that the affinity of the channel for AgTx2 increased during persistent binding and decreased during persistent dissociation. We propose a four-state model, including high- and low-affinity states of the channel, with relevant rate constants. An induced-fit pathway was dominant and accelerated binding by 400 times. This is the first analytical imaging of scorpion toxin binding in real time, which is applicable to various biological dynamics including channel ligands, DNA-modifier proteins, and antigen-antibody complexes. | |||||||||
| 権利 | ||||||||||
| 権利情報 | Copyright © 2019The Authors, somerights reserved;exclusive licenseeAmerican Associationfor the Advancementof Science. No claim tooriginal U.S. GovernmentWorks. Distributedunder a CreativeCommons AttributionNonCommercialLicense 4.0 (CC BY-NC). | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 関連URI | ||||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | https://advances.sciencemag.org/content/5/7/eaax0495 | |||||||||
| 関連名称 | https://advances.sciencemag.org/content/5/7/eaax0495 | |||||||||
