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新規病原ウイルス単離の試み
https://doi.org/10.24517/00054886
https://doi.org/10.24517/00054886f15a1e83-251d-4242-a029-895861d7a351
名前 / ファイル | ライセンス | アクション |
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ME-PR-MURAMATSU-M-kaken 2016-4p.pdf (220.7 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2019-07-11 | |||||
タイトル | ||||||
タイトル | 新規病原ウイルス単離の試み | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A trial to isolate novel pathogenic viruses | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00054886 | |||||
ID登録タイプ | JaLC | |||||
著者別表示 |
Muramatsu, Masamichi
× Muramatsu, Masamichi |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医薬保健研究域医学系 | |||||
書誌情報 |
平成27(2015)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書 en : 2015 Fiscal Year Final Research Report 巻 2014-04-01 - 2016-03-31, p. 4p., 発行日 2016-05-31 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 日和見感染時に病原性を示すウイルスのうち環状DNAをゲノムにもつものが特にポリオーマウイルスで報告されている。そこでDNA抽出産物から環状DNAを強力に増幅する手法で臨床検体中に新規病原体DNAが含まれるかを検討した。既存のパピローマウイルスは期待通り増幅されたが、新種のウイルスはみつからなかった。しかし由来不明の環状DNAを見つけた。由来不明DNA同定は病原性は不明だが一定の成果と言える。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Recently, many novel polyomaviruses have been identified as a candidate pathogenic virus that is responsible for particular diseases in immune compromised patients. This study has tried to isolate novel viruses responsible for a disease by rolling cycle amplification which can strongly amplify viral circular genomic DNA. Using test samples, we set up a condition where we can amplify a very small amount of circular DNA from total DNA sample. Then, we tested series of healthy- and disease- derived samples to amplify circular DNA. Most of amplified DNA are either artifacts or mitochondrial DNA. Skin samples provide amplification of MCV polyomavirus, that is consistent with previous published reports. However, we do not find a novel polyomavirus. Origin unknown circular DNA are also found, which is a clear outcome of this study, in spit of few information of pathogenicity. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:26670223, 研究期間(年度):2014-04-01 - 2016-03-31 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:研究課題「新規病原ウイルス単離の試み」課題番号:26670223 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26670223/26670223seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=20359813 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=20359813 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26670223/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26670223/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26670223/26670223seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26670223/26670223seika/ |