{"_buckets": {"deposit": "f88a176d-ed73-47c5-acd3-fa8fe3e3c5ba"}, "_deposit": {"created_by": 18, "id": "50567", "owners": [18], "pid": {"revision_id": 0, "type": "depid", "value": "50567"}, "status": "published"}, "_oai": {"id": "oai:kanazawa-u.repo.nii.ac.jp:00050567", "sets": ["2830"]}, "author_link": ["91488", "20737"], "item_9_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-04-18", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "2p.", "bibliographicVolumeNumber": "2001-2003", "bibliographic_titles": [{"bibliographic_title": "平成15(2003)年度 科学研究費補助金 基盤研究(C) 研究成果報告書"}, {"bibliographic_title": "2003 Fiscal Year Final Research Report Summary", "bibliographic_titleLang": "en"}]}]}, "item_9_creator_33": {"attribute_name": "著者別表示", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mori, Hirofumi"}], "nameIdentifiers": [{"nameIdentifier": "20737", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90019604", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90019604"}, {"nameIdentifier": "90019604", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000090019604"}]}]}, "item_9_description_21": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "片側性パーキンソンモデルラットを一側の線条体に6-OHDAを注入して作成し、迷走神経下神経節移植の効果について検討した。迷走神経下神経節移植は神経毒を注入してから2週後に行い、ドーパミンD_1、D_2レセプター及びドーパミントランスポータをそれぞれ[^3H]SCH23390、[^3H]YM-09151-2、[^3H]GBR12935を用いたインビトロオートラジオグラフィ法にて評価した。非特異的結合は[+]-ブタクラモールあるいは大量のGBR12935を用いて測定し、6-OHDA注入側と対側線条体の放射能比を指標とした。その結果、パーキンソンモデルラットにてドーパミントランスポータの著明な減少を認めた。移植ラットにてドーパミントランスポータとD_1レセプターは変化を認めなかったのに対し、D_2レセプターの有意な上昇を認めた。これらの結果より、迷走神経下神経節移植はドーパミントランスポータの改善には効果がないのに対し、6-OHDA注入側線条体のD_2レセプターのアップレギュレーションを誘導することが示された。\n痴呆疾患の分子イメージング剤としてシナプス小胞膜アセチルコリントランスポーターに注目し、ラット脳を用いた薬物阻害実験にて3種のヨードベサミコール類似物質の光学異性体((-)oIV,(-)mIV,(-)vesamicol,(+)oIV,(+)raIV,(+)vesamicol)についてベサミコールトランスポーターとシグマ(α1,α2)レセプターの結合の程度を比較した。その結果,(-)[^\u003c125\u003eI]-oIVは以前に報告した(-)-mIVよりもベサミコールトランスポーターに選択的に結合するのみならず、シグマレセプターとの結合が弱いため、PETあるいはSPECTによるアセチルコリントランスポーターのイメージング剤として有望であることが示された。さらに,このイメージング剤の改良とともに,他のアセチルコリン神経系の分子イメージング薬剤の合成について検討中である。", "subitem_description_type": "Abstract"}, {"subitem_description": "Parkinsonian rats were stereotaxically infused 6-OHDA into the unilateral mesostriatal dopamine pathway and measured apomorphine-induced rotational behavior. Controls were infused saline instead of neurotoxin. Animals were divided into transplantation(TP) and sham-operated(S) groups. Ipsilateral vagal nodosal ganglion was surgically removed horn die neck and stereotaxically transplanted as nerve fragments into the ipsilateral striatum 2 weeks after nerotoxin infusion Sequential brain sections underwent autoradiography of dopaminergic system 2 weeks after operation. Densities of dopamine D1,D2 receptors and dopamine transporter(DAT) were assessed by in-vitro autoradiography using[^3H]SCH23390,[^3H]YM-09151-2 and[^3H]GBR12935. Significant DAT decrease in striatum was shown in the model animals compared with controls(P\u003c0.0001). D_2 receptor in the ipsilateral striatum increased significantly in transplanted animals compared with sham-operated animals(S/R-,0.97±0.08;TP/R-,1.08±0.08;p=0.039 and S/R+,1.06±0.06;TP/R+,1.19±0.16;p=0.023), whereas DAT and D_1 receptor showed no significant changes. Our results suggest that vagal autotransplantation induces upregulation of D_2 receptor in the ipsilateral striatum. Autotransplantation showed no significant effect on DAT, although DAT imaging is a sensitive test for dopaminergic damage. We need hurther study on dopaminergic system imaging after neural transplantation.\nWe evaluated the potencies of radioiodimted(-)-o-iodovesamicol [(-)-oIV] as a selective vesicular acetylcholine tmnsporter(VAChT) mapping agen. (-)-[^\u003c125\u003e1] oIV exhibited significant accumulationin rat brain. The regional brain distribution of radioactivity was similar for both (-)-[^\u003c125\u003e1] oIV and [^3H]vesamicol. The accumulation of (-)-[^\u003c125\u003e1] oIV in the brain was significant reduced by post-administration of unlabeled vesamicol(0.5μmol/kg^\u003c-1\u003e) and(-)-[^\u003c125\u003e1] oIV μmol/kg-1] in the other hand, the post-administration of sigma ligands hardy affected the accumulation of (-)-[^\u003c125\u003e1] oIV in die brain. These studies showed that (-)-[^\u003c125\u003e1] oIV, as well as [^3H]vesamicol, bound to VAChT with high affinity in die rat brain. Funhemore, (-)-[^\u003c125\u003e1] oIV binding in the ipsilateral cortex to the lesion was significantly reduced by 17.0%, compared with that in the contralateral cortex in a unilateral NBM-lesioned rat. These results suggested that radioiodimted(-)-[^\u003c125\u003e1] oIV may potentially be useful for the diagnosis of cholinergic neurodegenerative disorders.", "subitem_description_type": "Abstract"}]}, "item_9_description_22": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "研究課題/領域番号:13670925, 研究期間(年度):2001-2003", "subitem_description_type": "Other"}, {"subitem_description": "出典：研究課題「分子イメージングによる痴呆疾患の客観的診断・治療効果判定に関する基礎的研究」課題番号13670925\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-13670925/136709252003kenkyu_seika_hokoku_gaiyo/）を加工して作成", "subitem_description_type": "Other"}]}, "item_9_description_5": {"attribute_name": "提供者所属", "attribute_value_mlt": [{"subitem_description": "金沢大学学際科学実験センター", "subitem_description_type": "Other"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24517/00056876", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_relation_28": {"attribute_name": "関連URI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://kaken.nii.ac.jp/search/?qm=90019604"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/search/?qm=90019604", "subitem_relation_type_select": "URI"}}, {"subitem_relation_name": [{"subitem_relation_name_text": "https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670925/"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670925/", "subitem_relation_type_select": "URI"}}, {"subitem_relation_name": [{"subitem_relation_name_text": "https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-13670925/136709252003kenkyu_seika_hokoku_gaiyo/"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-13670925/136709252003kenkyu_seika_hokoku_gaiyo/", "subitem_relation_type_select": "URI"}}]}, "item_9_version_type_25": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "森, 厚文"}], "nameIdentifiers": [{"nameIdentifier": "91488", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90019604", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90019604"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-04-11"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AD-PR-MORI-H-kaken 2015-2p.pdf", "filesize": [{"value": "91.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 91600.0, "url": {"label": "AD-PR-MORI-H-kaken 2015-2p.pdf", "url": "https://kanazawa-u.repo.nii.ac.jp/record/50567/files/AD-PR-MORI-H-kaken 2015-2p.pdf"}, "version_id": "74218dbc-d3a8-4945-a7dd-312f94d97332"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "痴呆疾患", "subitem_subject_scheme": "Other"}, {"subitem_subject": "核医学診断", "subitem_subject_scheme": "Other"}, {"subitem_subject": "放射性医薬品", "subitem_subject_scheme": "Other"}, {"subitem_subject": "モデル動物", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Radiopharmaceutical", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Dementia", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Receptor", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Transporter", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Neurotransplantation", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Radiopharmaceutical", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "分子イメージングによる痴呆疾患の客観的診断・治療効果判定に関する基礎的研究", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "分子イメージングによる痴呆疾患の客観的診断・治療効果判定に関する基礎的研究"}, {"subitem_title": "Fundamental Study on objective diagnosis and therapy effect decision of the dementia by the molecular imaging", "subitem_title_language": "en"}]}, "item_type_id": "9", "owner": "18", "path": ["2830"], "permalink_uri": "https://doi.org/10.24517/00056876", "pubdate": {"attribute_name": "公開日", "attribute_value": "2022-04-11"}, "publish_date": "2022-04-11", "publish_status": "0", "recid": "50567", "relation": {}, "relation_version_is_last": true, "title": ["分子イメージングによる痴呆疾患の客観的診断・治療効果判定に関する基礎的研究"], "weko_shared_id": -1}