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酸素輸送担体を介した筋細胞独自のミトコンドリア活性化機序
https://doi.org/10.24517/00057951
https://doi.org/10.24517/00057951ae0326aa-60db-47d1-b192-a936fc2d9b34
名前 / ファイル | ライセンス | アクション |
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HU-PR-MASUDA-K-kaken 2021-11p.pdf (369.2 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2022-02-17 | |||||
タイトル | ||||||
タイトル | 酸素輸送担体を介した筋細胞独自のミトコンドリア活性化機序 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Facilitation of mitochondrial respiration mediated by oxygen transporter in myocytes | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00057951 | |||||
ID登録タイプ | JaLC | |||||
著者別表示 |
Masuda, Kazumi
× Masuda, Kazumi |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学人間社会研究域人間科学系 | |||||
書誌情報 |
令和2(2020)年度 科学研究費補助金 基盤研究(B) 研究成果報告書 en : 2020 Fiscal Year Final Research Report 巻 2017-04-01 - 2021-03-31, p. 11p., 発行日 2021-06-18 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 本研究では筋細胞に特異的に存在するミオグロビン(Mb)がミトコンドリア(Mito)に及ぼす相互作用の機序を明らかにするため、Mitoの内部にMbが存在するかどうかを検証した。ラット腓腹筋から単離したMitoをショ糖密度勾配遠心分離法やProtection Assayによって、外膜/内膜/膜間腔/マトリックスに分画し、分析を重ねた結果、Mito に内在するMbは膜間腔と内膜に局在する可能性を示した。持久性トレーニングによって腓腹筋のMitoに内在するMb量は変化しなかったが、Mito内部へのMb輸送が能動的な機構である可能性が示された。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Recently, the muscle-specific protein myoglobin (Mb) has been reported to interact with complex IV to augment mitochondrial (Mito) respiratory capacity in skeletal muscles. The present study has aimed at to clarify the localization of Mb within Mito. The isolated Mit was treated with the sucrose density-gradient centrifugation or proteinase K (PK), osmotic shock (OS), and TriX in order to digest proteins on the outer membrane and in the intramembrane. Combining treatment with PK, OS and TriX allowed immunoblotting detection of the Mito proteins localized in specific regions of Mit. Mb was detected with either PK or OS treatment, , suggesting that Mb associated with the inner membrane (intramembrane side, not matrix side). The endurance training increased Mb content but did not change Mb content in the Mito. However, the observation might imply that the dynamic flux of Mb from cytosol to Mito has greater importance than just the amount found in Mito. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:17H02158, 研究期間(年度):2017-04-01 - 2021-03-31 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「酸素輸送担体を介した筋細胞独自のミトコンドリア活性化機序」研究成果報告書 課題番号17H02158 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17H02158/17H02158seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=50323283 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=50323283 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H02158/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H02158/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17H02158/17H02158seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17H02158/17H02158seika/ |