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Solubilization and Properties of Formate Dehydrogenase and Cytochrome b1 from Escherichia coli
https://doi.org/10.24517/00060073
https://doi.org/10.24517/00060073c1594861-1ab0-4e80-8687-c8a71cf5cf4d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SC-PR-ITAGAKI-E-52_131.pdf (865.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2020-12-03 | |||||||
| タイトル | ||||||||
| タイトル | Solubilization and Properties of Formate Dehydrogenase and Cytochrome b1 from Escherichia coli | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00060073 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Itagaki, Eiji
× Itagaki, Eiji× Fujita, Takeshi× Sato, Ryo |
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| 著者別表示 |
板垣, 英治
× 板垣, 英治
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| 書誌情報 |
The Journal of Biochemistry 巻 52, 号 2, p. 131-141, 発行日 1962 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0021-924X | |||||||
| NCID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00694073 | |||||||
| 出版者 | ||||||||
| 出版者 | The Japanese Biochemical Society | |||||||
| 出版者（別名） | ||||||||
| 出版者 | 日本生化学会 | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | A preparation containing formate dehydrogenase, cytochrome b1 and nitrate reductas_??_ was solubilized and partially purified from the particulate fraction of E. coli cells growr_??_ anaerobically under the conditions favorable for nitrate respiration. Some properties o_??_ formate dehydrogenase and cytochrome b1 in this purified preparation were investigated It was suggested that formate dehydrogenase is a metalloflavoprotein with essential sulf_??_ hydryl groups. Its activity was strongly bu_??_ reversibly inhibited by molecular oxyge_??_ The prosthetic group of cytochrome b1 was decisively identified as protoheme, and it was found that the cytochrome is autoxidizable. The reduction of cytochrome b1 by formate was shown to require, in addition to formate dehydrogenase, a lipid-soluble factor which could be replaced by vitamin K3 in the solubilized system. We are indefted to Drs. H. Maeno and S. Sakakibara for generous gifts of crude snake venonand crystalline protohemin, respectively. |
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| 権利 | ||||||||
| 権利情報 | Copyright © The Japanese Biochemical Society 日本生化学会 | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | http://www.jbsoc.or.jp/ | |||||||
| 関連名称 | http://www.jbsoc.or.jp/ | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://academic.oup.com/jb | |||||||
| 関連名称 | https://academic.oup.com/jb | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.jstage.jst.go.jp/browse/biochemistry1922/-char/ja | |||||||
| 関連名称 | https://www.jstage.jst.go.jp/browse/biochemistry1922/-char/ja | |||||||
