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Conductance selectivity of Na+ across the K+ channel via Na+ trapped in a tortuous trajectory
https://doi.org/10.24517/00061503
https://doi.org/10.24517/00061503cfbd27d6-f948-4240-9d02-44e977699421
| 名前 / ファイル | ライセンス | アクション |
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FR-PR-SUMIKAMA-T-2021-118.pdf (777.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2021-03-26 | |||||||
| タイトル | ||||||||
| タイトル | Conductance selectivity of Na+ across the K+ channel via Na+ trapped in a tortuous trajectory | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00061503 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Mita, Kenichiro
× Mita, Kenichiro× Sumikama, Takashi× Iwamoto, Masayuki× Matsuki, Yuka× Shigemi, Kenji× Oiki, Shigetoshi |
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| 著者別表示 |
炭竈, 享司
× 炭竈, 享司
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| 提供者所属 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 金沢大学ナノ生命科学研究所 | |||||||
| 書誌情報 |
PNAS en : Proceedings of the National Academy of Sciences of the United States of America 巻 118, 号 12, p. e2017168118, 発行日 2021-03-23 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0027-8424 | |||||||
| DOI | ||||||||
| 関連タイプ | isVersionOf | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1073/pnas.2017168118 | |||||||
| 出版者 | ||||||||
| 出版者 | National Academy of Sciences | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Ion selectivity of the potassium channel is crucial for regulating electrical activity in living cells; however, the mechanism underlying the potassium channel selectivity that favors large K+ over small Na+ remains unclear. Generally, Na+ is not completely excluded from permeation through potassium channels. Herein, the distinct nature of Na+ conduction through the prototypical KcsA potassium channel was examined. Single-channel current recordings revealed that, at a high Na+ concentration (200 mM), the channel was blocked by Na+, and this blocking was relieved at high membrane potentials, suggesting the passage of Na+ across the channel. At a 2,000 mM Na+ concentration, single-channel Na+ conductance was measured as one-eightieth of the K+ conductance, indicating that the selectivity filter allows substantial conduit of Na+. Molecular dynamics simulations revealed unprecedented atomic trajectories of Na+ permeation. In the selectivity filter having a series of carbonyl oxygen rings, a smaller Na+ was distributed off-center in eight carbonyl oxygen-coordinated sites as well as on-center in four carbonyl oxygen-coordinated sites. This amphipathic nature of Na+ coordination yielded a continuous but tortuous path along the filter. Trapping of Na+ in many deep free energy wells in the filter caused slow elution. Conversely, K+ is conducted via a straight path, and as the number of occupied K+ ions increased to three, the concerted conduction was accelerated dramatically, generating the conductance selectivity ratio of up to 80. The selectivity filter allows accommodation of different ion species, but the ion coordination and interactions between ions render contrast conduction rates, constituting the potassium channel conductance selectivity. | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Embargo Period 6 months | |||||||
| 権利 | ||||||||
| 権利情報 | Copyright © author | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.pnas.org/content/118/12/e2017168118 | |||||||
| 関連名称 | https://www.pnas.org/content/118/12/e2017168118 | |||||||
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| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.pnas.org/ | |||||||
| 関連名称 | https://www.pnas.org/ | |||||||
