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Cyclin D1 harboring the T286I mutation promotes oncogenic activation in endometrial cancer
https://doi.org/10.24517/00062677
https://doi.org/10.24517/000626775758fc8b-1aa0-4606-90c0-789d1ef0cf8e
| 名前 / ファイル | ライセンス | アクション |
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ME-PR-MAEDA-D-584.pdf (408.4 kB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2021-06-28 | |||||||
| タイトル | ||||||||
| タイトル | Cyclin D1 harboring the T286I mutation promotes oncogenic activation in endometrial cancer | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00062677 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Ikeda, Yuji
× Ikeda, Yuji× Oda, Katsutoshi× Hiraike-Wada, Osamu× Koso, Takahiro× Miyasaka, Aki× Kashiyama, Tomoko× Tanikawa, Michihiro× Sone, Kenbun× Nagasaka, Kazunori× Maeda, Daichi× Kawana, Kei× Nakagawa, Shunsuke× Fukayama, Masashi× Tetsu, Osamu× Fujii, Tomoyuki× Yano, Tetsu× Kozuma, Shiro |
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| 著者別表示 |
前田, 大地
× 前田, 大地
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| 提供者所属 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 金沢大学医薬保健研究域医学系 | |||||||
| 書誌情報 |
Oncology Reports 巻 30, 号 2, p. 584-588, 発行日 2013-06-03 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1021-335X | |||||||
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| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1791-2431 | |||||||
| NCID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA11016405 | |||||||
| DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.3892/or.2013.2515 | |||||||
| 出版者 | ||||||||
| 出版者 | Spandidos Publications | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Cyclin D1 is an important regulator of cell cycle progression. Phosphorylation of cyclin D1 at Thr286 by GSK3β triggers its nuclear export and cytoplasmic proteolysis via the 26S proteasome. Cyclin D1 overexpression is a common event in various types of human cancers; however, reports of mutations are extremely rare. We analyzed mutations of the cyclin D1 gene, CCND1, in 88 endometrial cancer tissue specimens and detected mutations in 2 cases (2.3%). Both were unreported mutations with substitution of threonine to isoleucine at codon 286 (T286I). These two tumors harbored coexisting mutations in K-ras, PIK3CA and/or PTEN and showed accumulation of cyclin D1 in the nucleus by immunohistochemistry. Furthermore, we analyzed the functions of mutant cyclin D1 (T286I) by luciferase assays, immunofluorescence, western blotting and clonogenic cell survival assays in HEK-293T cells. We found that exogenous mutant cyclin D1 (T286I) accumulated in the nuclei in HEK-293T cells, and that it inhibited the expression of pRb. Additionally, the number of colonies was increased by introduction of mutant cyclin D1 (T286I) compared to that of wild-type cyclin D1. In conclusion, we identified an unreported CCND1 mutation (T286I) in two endometrial cancers and revealed that the mutation was functional for inducing cell proliferation in human cells. | |||||||
| 権利 | ||||||||
| 権利情報 | Copyright © Spandidos Publications | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | http://www.spandidos-publications.com/or/ | |||||||
| 関連名称 | http://www.spandidos-publications.com/or/ | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.spandidos-publications.com/10.3892/or.2013.2515 | |||||||
| 関連名称 | https://www.spandidos-publications.com/10.3892/or.2013.2515 | |||||||
