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アイテム
IL-6 induction in desiccated corneal epithelium in vitro and in vivo
https://doi.org/10.24517/00062814
https://doi.org/10.24517/00062814c0cda5ba-a0cd-499d-8631-62928396d63a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
FR-PR-HIGUCHI-A-mv-v17-a261-higuchi.pdf (1.7 MB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2021-07-05 | |||||||
| タイトル | ||||||||
| タイトル | IL-6 induction in desiccated corneal epithelium in vitro and in vivo | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00062814 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Higuchi, Akihiro
× Higuchi, Akihiro× Kawakita, Tetsuya× Tsubota, Kazuo |
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| 著者別表示 |
樋口, 明弘
× 樋口, 明弘
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| 提供者所属 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 金沢大学先端科学・社会共創推進機構 | |||||||
| 書誌情報 |
Molecular Vision 巻 17, p. 2400-2406, 発行日 2011-09-13 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1090-0535 | |||||||
| NCID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA12037116 | |||||||
| 出版者 | ||||||||
| 出版者 | Molecular Vision | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Purpose: To investigate the effect of desiccation on secretion of inflammatory cytokines in corneal epithelial cells and in the rat desiccation model. Methods: A human corneal epithelial cell line (CEPI) was grown in keratinocyte growth medium 2 (KGM2) to approximately 80% confluence. The medium was aspirated and dishes were left for 0 to 30 min with the cover left open to dry the cells (short-term desiccation). After desiccation, KGM2 was added to the dishes and collected from the dishes 15 min later to measure the concentrations of cytokines in the medium by sandwich enzyme immunoassay (ELISA). Viability of the cells was estimated with alamer blue. To study the effect of long-term desiccation, cultivated cells on transwells were used. After dessiccation for up to 8 h, the viability of the cells and levels of cytokines in the culture medium were examined. The expression of cytokines in the cornea of the dry eye model rat was measured by real-time PCR. Results: Short-term dessication of CEPI cells significantly increased the interleukin (IL)-6 level and slightly increased the tumor necrosis factor (TNF)-α level. Anti-IL-6 antibody partially suppressed cell death caused by desiccation. Upon long-term desiccation, IL-6 and IL-8 levels were increased. In the dry eye model rats, the IL-6 mRNA level in the cornea significantly increased, whereas TNF-α mRNA level slightly increased. Conclusions: Desiccation induced IL-6 expression in corneal epithelial cells, suggesting that IL-6 participates in desiccation-induced cell death. © 2011 Molecular Vision. | |||||||
| 権利 | ||||||||
| 権利情報 | Copyright © Molecular Vision | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | http://www.molvis.org/molvis/ | |||||||
| 関連名称 | http://www.molvis.org/molvis/ | |||||||
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| 識別子タイプ | URI | |||||||
| 関連識別子 | http://www.molvis.org/molvis/volume17.html | |||||||
| 関連名称 | http://www.molvis.org/molvis/volume17.html | |||||||
