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Ligand-independent Tie2 dimers mediate kinase activity stimulated by high dose angiopoietin-1
https://doi.org/10.24517/00062983
https://doi.org/10.24517/00062983114009d0-e706-476b-b399-253760659ad8
| 名前 / ファイル | ライセンス | アクション |
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ME-PR-NAITO-H-12469.pdf (2.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2021-07-05 | |||||||||
| タイトル | ||||||||||
| タイトル | Ligand-independent Tie2 dimers mediate kinase activity stimulated by high dose angiopoietin-1 | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.24517/00062983 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| 著者 |
Yamakawa, Daishi
× Yamakawa, Daishi× Kidoya, Hiroyasu× Sakimoto, Susumu× Jia, Weizhen× Naito, Hisamichi× Takakura, Nobuyuki |
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| 著者別表示 |
内藤, 尚道
× 内藤, 尚道
× 高倉, 伸幸
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| 提供者所属 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 金沢大学医薬保健研究域医学系 | |||||||||
| 書誌情報 |
Journal of Biological Chemistry 巻 288, 号 18, p. 12469-12477, 発行日 2013-05-03 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0021-9258 | |||||||||
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| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 1083-351X | |||||||||
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| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA00251083 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1074/jbc.M112.433979 | |||||||||
| 出版者 | ||||||||||
| 出版者 | American Society for Biochemistry and Molecular Biology / Elsevier | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Tie2 is a receptor tyrosine kinase expressed on vascular endothelial cells (ECs). It has dual roles in promoting angiogenesis and stabilizing blood vessels, and it has been suggested that Tie2 forms dimers and/or oligomers in the absence of angiopoietin-1 (Ang1); however, the mechanism of ligand-independent dimerization of Tie2 and its biological significance have not been clarified. Using a bimolecular fluorescence complementation assay and a kinase-inactive Tie2 mutant, we show here that ligand-independent Tie2 dimerization is induced without Tie2 phosphorylation. Moreover, based on the fact that Tie1 never forms heterodimers with Tie2 in the absence of Ang1 despite having high amino acid sequence homology with Tie2, we searched for ligand-independent dimerization domains of Tie2 by reference to the difference with Tie1. We found that the YIA sequence of the intracellular domain of Tie2 corresponding to the LAS sequence in Tie1 is essential for this dimerization. When the YIA sequence was replaced by LAS in Tie2 (Tie2YIA/LAS), ligand-independent dimer was not formed in the absence of Ang1. When activation of Tie2YIA/LAS was induced by a high dose of Ang1, phosphorylation of Tie2 was limited compared with wild-type Tie2, resulting in retardation of activation of Erk downstream of Tie2. Therefore, these data suggest that ligand-independent dimerization of Tie2 is essential for a strong response upon stimulation with high dose Ang1. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. | |||||||||
| 権利 | ||||||||||
| 権利情報 | copyright © American Society for Biochemistry and Molecular Biology | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 関連URI | ||||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | https://www.journals.elsevier.com/journal-of-biological-chemistry | |||||||||
| 関連名称 | https://www.journals.elsevier.com/journal-of-biological-chemistry | |||||||||
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| 識別子タイプ | URI | |||||||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S0021925819333125?via%3Dihub | |||||||||
| 関連名称 | https://www.sciencedirect.com/science/article/pii/S0021925819333125?via%3Dihub | |||||||||
