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Fluorescence and bioluminescence imaging of angiogenesis in Flk1-Nano-lantern transgenic mice
https://doi.org/10.24517/00062990
https://doi.org/10.24517/00062990d097bf0c-1559-4934-8ebe-59ed0aed052c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ME-PR-NAITO-H-46597.pdf (12.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2021-07-05 | |||||||||
| タイトル | ||||||||||
| タイトル | Fluorescence and bioluminescence imaging of angiogenesis in Flk1-Nano-lantern transgenic mice | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.24517/00062990 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| 著者 |
Matsushita, Jun
× Matsushita, Jun× Inagaki, Shigenori× Nishie, Tomomi× Sakasai, Tomoki× Tanaka, Junko× Watanabe, Chisato× Mizutani, Ken-ichi× Miwa, Yoshihiro× Matsumoto, Ken× Takara, Kazuhiro× Naito, Hisamichi× Kidoya, Hiroyasu× Takakura, Nobuyuki× Nagai, Takeharu× Takahashi, Satoru× Ema, Masatsugu |
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| 著者別表示 |
内藤, 尚道
× 内藤, 尚道
× 高倉, 伸幸
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| 提供者所属 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 金沢大学医薬保健研究域医学系 | |||||||||
| 書誌情報 |
Scientific Reports 巻 7, p. 46597, 発行日 2017-04-20 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 2045-2322 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1038/srep46597 | |||||||||
| 出版者 | ||||||||||
| 出版者 | Nature Research / Nature Publishing Group | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Angiogenesis is important from normal development as well as from tumour growth. However, the molecular and cellular mechanisms underlying angiogenesis are not fully understood, partly because of the lack of a good animal model from imaging. Here, we report the generation of a novel transgenic (Tg) mouse that expresses a bioluminescent reporter protein, Nano-lantern, under the control of Fetal liver kinase 1 (Flk1). Flk1-Nano-lantern BAC Tg mice recapitulated endogenous Flk1 expression in endothelial cells and lymphatic endothelial cells during development and tumour growth. Importantly, bioluminescence imaging of endothelial cells from the aortic rings of Flk1-Nano-lantern BAC Tg mice enabled us to observe endothelial sprouting from 18 hr without any detectable phototoxicity. Furthermore, Flk1-Nano-lantern BAC Tg mice achieved time-lapse luminescence imaging of tumour angiogenesis in freely moving mice with implanted tumours. Thus, this transgenic mouse line contributes a unique model to study angiogenesis within both physiological and pathological contexts. | |||||||||
| 権利 | ||||||||||
| 権利情報 | copyright © Nature Research | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 関連URI | ||||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | http://www.nature.com/srep/index.html | |||||||||
| 関連名称 | http://www.nature.com/srep/index.html | |||||||||
| 関連URI | ||||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | https://www.nature.com/articles/srep46597 | |||||||||
| 関連名称 | https://www.nature.com/articles/srep46597 | |||||||||
