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Synaptically driven endocannabinoid release requires Ca2+- assisted metabotropic glutamate receptor subtype 1 to phospholipase C β4 signaling cascade in the cerebellum
https://doi.org/10.24517/00064866
https://doi.org/10.24517/0006486627eca66f-0491-4013-9479-91e83e196df7
| 名前 / ファイル | ライセンス | アクション |
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ME-PR-SYOSAKU-T-6826.pdf (602.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2022-02-04 | |||||||||
| タイトル | ||||||||||
| タイトル | Synaptically driven endocannabinoid release requires Ca2+- assisted metabotropic glutamate receptor subtype 1 to phospholipase C β4 signaling cascade in the cerebellum | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.24517/00064866 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| 著者 |
Maejima, Takashi
× Maejima, Takashi× Oka, Saori× Hashimotodani, Yuki× Ohno-Shosaku, Takako× Aiba, Atsu× Wu, Dianqing× Waku, Keizo× Sugiura, Takayuki× Kano, Masanobu |
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| 著者別表示 |
少作, 隆子
× 少作, 隆子
× 狩野, 方伸
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| 提供者所属 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 金沢大学医薬保健研究域保健学系 | |||||||||
| 書誌情報 |
Journal of Neuroscience en : The official journal of the Society for Neuroscience 巻 25, 号 29, p. 6826-6835, 発行日 2005-07-20 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0270-6474 | |||||||||
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| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 1529-2401 | |||||||||
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| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA10620404 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1523/JNEUROSCI.0945-05.2005 | |||||||||
| 出版者 | ||||||||||
| 出版者 | Society for Neuroscience | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Endocannabinoids mediate retrograde signaling and modulate synaptic transmission in various regions of the CNS. Depolarization-induced elevation of intracellular Ca2+ concentration causes endocannabinoid-mediated suppression of excitatory/inhibitory synaptic transmission. Activation of G q/11-coupled receptors including group I metabotropic glutamate receptors (mGluRs) also causes endocannabinoid-mediated suppression of synaptic transmission. However, precise mechanisms of endocannabinoid production initiated by physiologically relevant synaptic activity remain to be determined. To address this problem, we made whole-cell recordings from Purkinje cells (PCs) in mouse cerebellar slices and examined their excitatory synapses arising from climbing fibers (CFs) and parallel fibers (PFs). We first characterized three distinct modes to induce endocannabinoid release by analyzing CF to PC synapses. The first mode is strong activation of mGluR subtype 1 (mGluR1)-phospholipase C (PLC) β4 cascade without detectable Ca 2+ elevation. The second mode is Ca2+ elevation to a micromolar range without activation of the mGluR1-PLC/34 cascade. The third mode is the Ca2+-assisted mGluR1-PLCβ4 cascade that requires weak mGluR1 activation and Ca2+ elevation to a submicromolar range. By analyzing PF to PC synapses, we show that the third mode is essential for effective endocannabinoid release from PCs by excitatory synaptic activity. Furthermore, our biochemical analysis demonstrates that combined weak mGluR1 activation and mild depolarization in PCs effectively produces 2-arachidonoylglycerol (2-AG), a candidate of endocannabinoid, whereas either stimulus alone did not produce detectable 2-AG. Our results strongly suggest that under physiological conditions, excitatory synaptic inputs to PCs activate the Ca2+-assisted mGluR1-PLCβ4 cascade, and thereby produce 2-AG, which retrogradely modulates synaptic transmission to PCs. Copyright © 2005 Society for Neuroscience. | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | This work was supported by Grants-in-Aid for Scientific Research and Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Sports, Culture, Science and Technology ofJapan. This work was alsosupported by theJapan Society for the Promotion of Science (JSPS) and the Toyota RIKEN Foundation. T.M. was a recipient of JSPS Research Fellowships for Young Scientists and the Research Aid of Inoue Foundation for Science. We thank S. Arai for 2-AG estimation and Drs. K. Hashimoto and T. Tabata for comments on this work. | |||||||||
| 権利 | ||||||||||
| 権利情報 | Copyright © 2005 Society for Neuroscience | |||||||||
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| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
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| 識別子タイプ | URI | |||||||||
| 関連識別子 | http://www.jneurosci.org/ | |||||||||
| 関連名称 | http://www.jneurosci.org/ | |||||||||
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| 識別子タイプ | URI | |||||||||
| 関連識別子 | https://www.jneurosci.org/content/25/29/6826 | |||||||||
| 関連名称 | https://www.jneurosci.org/content/25/29/6826 | |||||||||
