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Rapid screening by cell-based fusion assay for identifying novel antivirals of glycoprotein B-mediated herpes simplex virus type 1 infection
https://doi.org/10.24517/00067075
https://doi.org/10.24517/00067075c4bc6a7a-2c5e-4285-85fe-af2b85da24cf
| 名前 / ファイル | ライセンス | アクション |
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PH-PR-FURUKAWA-A-39-1897.pdf (534.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2022-09-12 | |||||||
| タイトル | ||||||||
| タイトル | Rapid screening by cell-based fusion assay for identifying novel antivirals of glycoprotein B-mediated herpes simplex virus type 1 infection | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00067075 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Maeda, Naoyoshi
× Maeda, Naoyoshi× Furukawa, Atsushi× Kakita, Kosuke× Anada, Masahiro× Hashimoto, Shunichi× Matsunaga, Shigeki× Kuroki, Kimiko× Ose, Toyoyuki× Kato, Akihisa× Arii, Jun× Kawaguchi, Yasushi× Arase, Hisashi× Maenaka, Katsumi |
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| 著者別表示 |
古川, 敦
× 古川, 敦
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| 提供者所属 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 金沢大学医薬保健研究域薬学系 | |||||||
| 書誌情報 |
Biological and Pharmaceutical Bulletin 巻 39, 号 11, p. 1897-1902, 発行日 2016-11-01 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0918-6158 | |||||||
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| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1347-5215 | |||||||
| NCID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA10885497 | |||||||
| DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1248/bpb.b16-00533 | |||||||
| 出版者 | ||||||||
| 出版者 | Pharmaceutical Society of Japan | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Herpes simplex virus type 1 (HSV-1) is a causative agent for a variety of diseases. Although antiherpetic drugs such as acyclovir have been developed to inhibit virus replication through interaction with DNA kinases, their continuous administration leads to an increase in the frequency of drug-resistant HSV-1, which is an important clinical issue that requires urgent solution. Recently, we reported that the sialylated O-linked sugar T antigen (sTn) and its attached peptide region (O-glycosylated sTn peptide) derived from the HSV-1 glycoprotein B (gB) protein inhibited HSV-1 infection by specifically targeting paired immunoglobulin-like type 2 receptor alpha (PILRα) in vitro. In this study, to further identify novel inhibitors of gB-mediated HSV-1 infection in vitro, we established a cell-based fusion assay for rapid drug screening. Chinese hamster ovary (CHO) cells were transfected with expression plasmids for HSV-1 gB, gD, gH, and gL, and T7 RNA polymerase, and were designated as the effector cells. The CHO-K1 cells stably expressing PILRα were transfected with the expression plasmid for firefly luciferase under the T7 promoter, and were designated as the target cells. The effector and target cells were co-cultured, and luminescence was measured when both cells were successfully fused. Importantly, we found that cell-to-cell fusion was specifically inhibited by Oglycosylated sTn peptide in a dose dependent manner. Our results suggested that this virus-free cell-based fusion assay system could be a useful and promising approach to identify novel inhibitors of gB-mediated HSV-1 infection, and will aid in the development of antiviral therapeutic strategies for HSV-1-associated diseases. © 2016 The Pharmaceutical Society of Japan. | |||||||
| 権利 | ||||||||
| 権利情報 | Copyright © 2016 Pharmaceutical Society of Japan | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.jstage.jst.go.jp/browse/bpb/-char/ja/ | |||||||
| 関連名称 | https://www.jstage.jst.go.jp/browse/bpb/-char/ja/ | |||||||
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| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.pharm.or.jp/publication/ | |||||||
| 関連名称 | https://www.pharm.or.jp/publication/ | |||||||
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| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.pharm.or.jp/ | |||||||
| 関連名称 | https://www.pharm.or.jp/ | |||||||
