Involvement of β-defensin 130 (DEFB130) in the macrophage microbicidal mechanisms for killing Plasmodium falciparum

古川, 敦; Terkawi, Mohamad Alaa; Takano, Ryo; Furukawa, Atsushi; Murakoshi, Fumi; Kato, Kentaro

doi:10.1038/srep41772

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Involvement of β-defensin 130 (DEFB130) in the macrophage microbicidal mechanisms for killing Plasmodium falciparum

https://doi.org/10.24517/00067084

名前 / ファイル	ライセンス	アクション
PH-PR-FURUKAWA-A-7-4177.pdf (2.7 MB)

Item type

学術雑誌論文 / Journal Article(1)

公開日

2022-09-12

タイトル

Involvement of β-defensin 130 (DEFB130) in the macrophage microbicidal mechanisms for killing Plasmodium falciparum

言語

eng

資源タイプ

資源タイプ識別子

http://purl.org/coar/resource_type/c_6501

資源タイプ

journal article

ID登録

10.24517/00067084

ID登録タイプ

JaLC

著者

Terkawi, Mohamad Alaa

Takano, Ryo
Furukawa, Atsushi

WEKO 106943
e-Rad 30727699

	Furukawa, Atsushi

Search repository

Murakoshi, Fumi

Kato, Kentaro

著者別表示

古川, 敦

提供者所属

内容記述タイプ

Other

内容記述

金沢大学医薬保健研究域薬学系

書誌情報

Scientific Reports

巻 7, p. 41772, 発行日 2017-02-09

ISSN

収録物識別子タイプ

ISSN

収録物識別子

2045-2322

DOI

関連識別子

10.1038/srep41772

出版者

Nature Publishing Group

抄録

内容記述タイプ

Abstract

内容記述

Understanding the molecular defense mechanism of macrophages and identifying their effector molecules against malarial parasites may provide important clues for the discovery of new therapies. To analyze the immunological responses of malarial parasite-induced macrophages, we used DNA microarray technology to examine the gene profile of differentiated macrophages phagocytizing Plasmodium falciparum-parasitized erythrocytes (iRBC). The transcriptional gene profile of macrophages in response to iRBCs represented 168 down-regulated genes, which were mainly involved in the cellular immune response, and 216 upregulated genes, which were involved in cellular proteolysis, growth, and adhesion. Importantly, the specific upregulation of β-defensin 130 (DEFB130) in these macrophages suggested a possible role for DEFB130 in malarial parasite elimination. Differentiated macrophages phagocytizing iRBCs exhibited an increase in intracellular DEFB130 levels and DEFB130 appeared to accumulate at the site of iRBC engulfment. Transfection of esiRNA-mediated knockdown of DEFB130 into macrophages resulted in a remarkable reduction in their antiplasmodial activity in vitro. Furthermore, DEFB130 synthetic peptide exhibited a modest toxic effect on P. falciparum in vitro and P. yoelii in vivo, unlike scrambled DEFB130 peptide, which showed no antiplasmodial activity. Together, these results suggest that DEFB130 might be one of the macrophage effector molecules for eliminating malarial parasites. Our data broaden our knowledge of the immunological response of macrophages to iRBCs and shed light on a new target for therapeutic intervention. © The Author(s) 2017.

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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

著者版フラグ

出版タイプ

VoR

出版タイプResource

http://purl.org/coar/version/c_970fb48d4fbd8a85