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Effect of topoisomerase inhibitors on the formation of replication protein A-foci in nuclei reconstituted in Xenopus egg extract
http://hdl.handle.net/2297/14518
http://hdl.handle.net/2297/1451849c365ed-9d66-456c-aa01-2a5b18e7fa2b
| 名前 / ファイル | ライセンス | アクション |
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TE-PR-KOBAYASHI-T-296.pdf (439.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Effect of topoisomerase inhibitors on the formation of replication protein A-foci in nuclei reconstituted in Xenopus egg extract | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Kobayashi, Takayuki
× Kobayashi, Takayuki× Tada, Shusuke× Tsuyama, Takashi× Murofushi, Hiromu× Seki, Masayuki× Enomoto, Takemi |
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| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学大学院自然科学研究科 信頼性システム工学 | |||||
| 書誌情報 |
Journal of Health Science 巻 48, 号 3, p. 296-301, 発行日 2002-06-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1344-9702 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11316464 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1248/jhs.48.296 | |||||
| 出版者 | ||||||
| 出版者 | 日本薬学会 = Pharmaceutical Society of Japan | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Some DNA topoisomerase inhibitors induce cleavage of DNA strands and lead to cell death. Therefore the inhibitors are utilized for chemotherapy against cancer. The treatment, however, can cause severe side effects such as genetic modification of normal cells. Camptothecin (CPT) and etoposide (VP16) belong to this class of anticancer agents that inhibit DNA topoisomerase I and II, respectively. In the present study, we investigated whether treatment with CPT and VP16 induces DNA breaks in nuclei reconstituted in Xenopus egg extract by observing focus formation of replication protein A (RPA), which is thought to accumulate on loci of DNA lesions prior to DNA repair. Treatment with CPT caused formation of discrete RPA foci at a later stage of incubation of demembranated Xenopus sperm nuclei with egg extracts. The RPA foci increased with the amount of CPT. However, at 30 min, before or just starting the initiation of DNA replication, the discrete RPA foci in CPT-containing extract were not obvious, suggesting that DNA replication is necessary for the focus formation induced by CPT. On the other hand, discrete RPA foci were induced by VP16 even at 30 min. | |||||
| 権利 | ||||||
| 権利情報 | Copyright (c) 2002 by The Pharmaceutical Society of Japan | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jstage.jst.go.jp/article/jhs/48/3/48_296/_article/-char/en | |||||
| 関連URI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://dx.doi.org/10.1248/jhs.48.296 | |||||
