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ヒト血清アルブミンリン酸緩衝溶液の近赤外領域における分光学的特性およびその定量化の試み
http://hdl.handle.net/2297/28487
http://hdl.handle.net/2297/2848728818966-c3ce-4cd7-b351-e0a2ee25b985
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-03 | |||||
タイトル | ||||||
タイトル | ヒト血清アルブミンリン酸緩衝溶液の近赤外領域における分光学的特性およびその定量化の試み | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Near-infrared spectroscopy of human serum albumin and determination of its concentrations in phosphate buffer solutions | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
小川, 充洋
× 小川, 充洋× 山越, 健弘× 田中, 志信× 山越, 憲一 |
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書誌情報 |
生体医工学 : 日本エム・イー学会誌 = Transactions of the Japanese Society for Medical and Biological Engineering 巻 48, 号 3, p. 259-268, 発行日 2010-06-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-443X | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11633569 | |||||
出版者 | ||||||
出版者 | 日本生体医工学会 = Japanese Society for medical and Bilogical Engineering | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Near-infrared differential absorbance spectra from pure water have been measured for phosphate buffer solutions (pH = 7.4) of human serum albumin (HSA) in the wavelength range of 750-2500nm. The peaks that follow HSA concentration can be observed in near-infrared differential absorbance spectra; in the 1650-1750nm and 2150-2350nm region. The near-infrared spectra in the 750-1350nm, 1550-1850nm and 2052-2500nm regions of the solutions have been subjected to partial least squares (PLS) regression analysis to make calibration models predicting the concentrations of the HSA. The calibration in the concentration range of 0-5g/dl has yielded a correlation coefficient of 0.999 and a standard error of prediction of 0.0283g/dl with latent variable of seven. This result suggests that the near-infrared differential spectra of HSA from pure water reference and its calibration model constructed by using PLS regression can pick up effectively the information about albumin contained in phosphate buffer solutions. This result can be a base of reagentless measurement of HSA in human serum and in vivo HSA measurement. | |||||
権利 | ||||||
権利情報 | Copyright (c) 2011 社団法人日本生体医工学会 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||