Biochemical and biophysical research communications
巻
299
号
5
ページ
697 - 702
発行年
2002-12-01
ISSN
0006-291X
DOI
https://doi.org/10.1016/s0006-291x(02)02692-x
出版者
Elsevier
抄録
c-Abl plays important roles in cellular response to DNA damage. However, possible roles for Arg (Abl-related gene) in DNA damage response are unknown. Here, we show that ionizing radiation (IR)-induced Rad51 focus formation is reduced in Arg-deficient cells generated from a chicken B cell line by targeted disruption. This is consistent with the findings that Arg-deficient cells display hypersensitivity to IR, elevated frequencies of IR-induced chromosomal aberrations, and reduced targeted integration frequencies. All of these abnormalities in DNA damage repair are also observed in ATM-deficient cells but not in c-Abl-deficient cells. Finally, we show that Arg interacts with and phosphorylates Rad51 in 293T cells. These results suggest that Arg plays a role in homologous recombinational (HR) DNA repair by phosphorylating Rad51.
Arg tyrosine kinase is involved in homologous recombinational DNA repair
CA-PR-YAMAMOTO-K-Li et al(BBRC.pdf
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