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セレノプロテインPを介した脂肪酸によるインスリン抵抗性回復機序の解明
https://doi.org/10.24517/00054584
https://doi.org/10.24517/00054584fca36eef-952f-4081-a385-64ff11f3066a
名前 / ファイル | ライセンス | アクション |
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ME-PR-TAKESHITA-Y-kaken 2020-13p.pdf (203.2 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2021-04-16 | |||||
タイトル | ||||||
タイトル | セレノプロテインPを介した脂肪酸によるインスリン抵抗性回復機序の解明 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Mechanism of insulin resistance by fatty acids via selenoprotein P | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00054584 | |||||
ID登録タイプ | JaLC | |||||
著者別表示 |
Takeshita, Yumie
× Takeshita, Yumie |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学附属病院内分泌・代謝内科 | |||||
書誌情報 |
令和1(2019)年度 科学研究費補助金 基盤研究(C) 研究成果報告書 en : 2019 Fiscal Year Final Research Report 巻 2016-04-01 - 2020-03-31, p. 13p., 発行日 2020-05-22 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | パルミチン酸 (PA)及びエイコサペンタエン酸 (EPA)がインスリン抵抗性誘導セレノプロテインP (SeP)発現に及ぼす影響を検討。H4IIEC肝細胞において、Sepp1遺伝子発現はPAにより亢進し、EPAにより減少。SEPP1プロモーター上の応答配列はPA及びEPAに対して共通で、Sterol regulatory element (SRE)-likeな配列を有する。PAはSREBP-1c経路非依存的にSeP発現を誘導、EPAはSREBP-1c経路依存的にSeP発現を抑制。EPAはSREBP-1cの不活化を介し肝臓でのSeP発現を抑制し、全身のインスリン抵抗性を改善する可能性がある。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Selenoprotein P (encoded by SELENOP in humans, Selenop in rat), a liver-derived secretory protein, induces resistance to insulin and VEGF in type 2 diabetes. The present findings demonstrate that EPA suppresses SELENOP expression by inactivating SREBP-1c in H4IIEC3 hepatocytes. Treatment with EPA caused concentration- and time-dependent reduction in SELENOP promoter activity. EPA activated AMPK; however, the inhibitory effect of EPA on SELENOP promoter activity was not canceled with an AMPK inhibitor compound C and dominant-negative AMPK transfection. Deletion mutant promoter assays and computational analysis of transcription factor-binding sites conserved among the species resulted in identification of a SRE-like site in the SELENOP promoter. A ChIP assay revealed that EPA decreases binding of SREBP-1c to the SELENOP promoter. Knockdown of Srebf1 resulted in a significant down-regulation of Selenop expression. SREBP-1c overexpression inhibited the suppressive effect of EPA. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:16K09739, 研究期間(年度):2016-04-01 - 2020-03-31 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「セレノプロテインPを介した脂肪酸によるインスリン抵抗性回復機序の解明」研究成果報告書 課題番号16K09739 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K09739/16K09739seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=40507042 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=40507042 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K09739/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K09739/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K09739/16K09739seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K09739/16K09739seika/ |