| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-03 |
| タイトル |
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タイトル |
Upregulation of anti-apoptotic factors in upper motor neurons after spinal cord injury in adult zebrafish |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
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|
ID登録 |
10.24517/00013898 |
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ID登録タイプ |
JaLC |
| その他のタイトル |
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|
値 |
ゼブラフィッシュ脊髄損傷後の上位運動ニューロンにおける抗アポトーシス因子の発現上昇について |
| 著者 |
Ogai, Kazuhiro
Hisano, Suguru
Mawatari, Kazuhiro
Sugitani, Kayo
Koriyama, Yoshiki
Nakashima, Hiroshi
Kato, Satoru
|
| 著者別表示 |
大貝, 和裕
馬渡, 一浩
杉谷, 加代
郡山, 恵樹
中島, 広志
加藤, 聖
|
| 書誌情報 |
Neurochemistry International
巻 61,
号 7,
p. 1202-1211,
発行日 2012-12-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0197-0186 |
| NCID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA0032399X |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.neuint.2012.08.015 |
| 出版者 |
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出版者 |
Elsevier |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Unlike mammals, fish motor function can recover within 6-8 weeks after spinal cord injury (SCI). The motor function of zebrafish is regulated by dual control; the upper motor neurons of the brainstem and motor neurons of the spinal cord. In this study, we aimed to investigate the framework behind the regeneration of upper motor neurons in adult zebrafish after SCI. In particular, we investigated the cell survival of axotomized upper motor neurons and its molecular machinery in zebrafish brain. As representative nuclei of upper motor neurons, we retrogradely labeled neurons in the nucleus of medial longitudinal fasciculus (NMLF) and the intermediate reticular formation (IMRF) using a tracer injected into the lesion site of the spinal cord. Four to eight neurons in each thin sections of the area of NMLF and IMRF were successfully traced at least 1-15 days after SCI. TUNEL staining and BrdU labeling assay revealed that there was no apoptosis or cell proliferation in the axotomized neurons of the brainstem at various time points after SCI. In contrast, axotomized neurons labeled with a neurotracer showed increased expression of anti-apoptotic factors, such as Bcl-2 and phospho-Akt (p-Akt), at 1-6 days after SCI. Such a rapid increase of Bcl-2 and p-Akt protein levels after SCI was quantitatively confirmed by western blot analysis. These data strongly indicate that upper motor neurons in the NMLF and IMRF can survive and regrow their axons into the spinal cord through the rapid activation of anti-apoptotic molecules after SCI. The regrowing axons from upper motor neurons reached the lesion site at 10-15 days and then crossed at 4-6 weeks after SCI. These long-distance descending axons from originally axotomized neurons have a major role in restoration of motor function after SCI. © 2012 Elsevier Ltd. All rights reserved. |
| 内容記述 |
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内容記述タイプ |
Other |
|
内容記述 |
Thesis of Kazuhiro Ogai / 大貝 和裕 博士論文 金沢大学医薬保健学総合研究科（保健学専攻） |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連URI |
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|
識別子タイプ |
URI |
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関連識別子 |
http://www.elsevier.com/locate/issn/01970186 |
ME-PR-OGAI-K-1202.pdf (1.7 MB)
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