| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-03 |
| タイトル |
|
|
タイトル |
Membrane-type 1 matrix metalloproteinase regulates fibronectin assembly and N-cadherin adhesion |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00014064 |
|
ID登録タイプ |
JaLC |
| 著者 |
Takino, Takahisa
Yoshimoto, Taisuke
Nakada, Mitsutoshi
Li, Zichen
Domoto, Takahiro
Kawashiri, Shuici
Sato, Hiroshi
|
| 著者別表示 |
滝野, 隆久
中田, 光俊
堂本, 貴寛
川尻, 秀一
佐藤, 博
|
| 書誌情報 |
Biochemical and Biophysical Research Communications
巻 450,
号 2,
p. 1016-1020,
発行日 2014-07-25
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1090-2104 |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11542044 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bbrc.2014.06.100 |
| 出版者 |
|
|
出版者 |
Academic Press |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Fibronectin matrix formation requires the increased cytoskeletal tension generated by cadherin adhesions, and is suppressed by membrane-type 1 matrix metalloproteinase (MT1-MMP). In a co-culture of Rat1 fibroblasts and MT1-MMP-silenced HT1080 cells, fibronectin fibrils extended from Rat1 to cell-matrix adhesions in HT1080 cells, and N-cadherin adhesions were formed between Rat1 and HT1080 cells. In control HT1080 cells contacting with Rat1 fibroblasts, cell-matrix adhesions were formed in the side away from Rat1 fibroblasts, and fibronectin assembly and N-cadherin adhesions were not formed. The role of N-cadherin adhesions in fibronectin matrix formation was studied using MT1-MMP-silenced HT1080 cells. MT1-MMP knockdown promoted fibronectin matrix assembly and N-cadherin adhesions in HT1080 cells, which was abrogated by double knockdown with either integrin β1 or fibronectin. Conversely, inhibition of N-cadherin adhesions by its knockdown or treatment with its neutralizing antibody suppressed fibronectin matrix formation in MT1-MMP-silenced cells. These results demonstrate that fibronectin assembly initiated by MT1-MMP knockdown results in increase of N-cadherin adhesions, which are prerequisite for further fibronectin matrix formation. © 2014 Elsevier Inc. All rights reserved. |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
CA-PR-TAKINO-T-1016.pdf (921.8 kB)
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