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Real-Time Fluorescence Imaging of the DNA Damage Repair Response during Mitosis
http://hdl.handle.net/2297/43946
http://hdl.handle.net/2297/439462d4d91ae-8f57-42db-be8b-154f15b651aa
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ME-PR-MIWA-S-661.pdf (973.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Real-Time Fluorescence Imaging of the DNA Damage Repair Response during Mitosis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Miwa, Shinji
× Miwa, Shinji× Yano, Shuya× Yamamoto, Mako× Matsumoto, Yasunori× Uehara, Fuminari× Hiroshima, Yukihiko× Toneri, Makoto× Murakami, Takashi× Kimura, Hiroaki× Hayashi, Katsuhiro× Yamamoto, Norio× Efimova, Elena V.× Tsuchiya, Hiroyuki× Hoffman, Robert M. |
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| 書誌情報 |
Journal of Cellular Biochemistry 巻 116, 号 4, p. 661-666, 発行日 2015-04-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0730-2312 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1002/jcb.25021 | |||||
| 出版者 | ||||||
| 出版者 | Wiley-Liss Inc. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The response to DNA damage during mitosis was visualized using real-time fluorescence imaging of focus formation by the DNA-damage repair (DDR) response protein 53BP1 linked to green fluorescent protein (GFP) (53BP1-GFP) in the MiaPaCa-2Tet-On pancreatic cancer cell line. To observe 53BP1-GFP foci during mitosis, MiaPaCa-2Tet-On 53BP1-GFP cells were imaged every 30 min by confocal microscopy. Time-lapse imaging demonstrated that 11.4 ± 2.1% of the mitotic MiaPaCa-2Tet-On 53BP1-GFP cells had increased focus formation over time. Non-mitotic cells did not have an increase in 53BP1-GFP focus formation over time. Some of the mitotic MiaPaCa-2Tet-On 53BP1-GFP cells with focus formation became apoptotic. The results of the present report suggest that DNA strand breaks occur during mitosis and undergo repair, which may cause some of the mitotic cells to enter apoptosis in a phenomenon possibly related to mitotic catastrophe. J. Cell. Biochem. 116: 661-666, 2015. © 2014 Wiley Periodicals, Inc. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
