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Analysis of 8-hydroxy-2'-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry
http://hdl.handle.net/2297/31391
http://hdl.handle.net/2297/31391e48269de-ede2-4037-a71f-7472a31f4bbb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
PH-PR-TORIBA-Y-173.pdf (187.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-04 | |||||
| タイトル | ||||||
| タイトル | Analysis of 8-hydroxy-2'-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Hosozumi, Chiemi
× Hosozumi, Chiemi× Toriba, Akira× Chuesaard, Thanyarat× Kameda, Takayuki× Tang, Ning× Hayakawa, Kazuichi |
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| 書誌情報 |
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 巻 893-894, p. 173-176, 発行日 2012-04-15 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1570-0232 | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11656178 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.jchromb.2012.02.043 | |||||
| 出版者 | ||||||
| 出版者 | Elsevier B.V. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a widely used noninvasive biomarker of oxidative stress. A selective, sensitive and rapid method for determining 8-OHdG in human urine was developed using hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) with electrospray ionization. 8-OHdG and isotopically labeled 8-OHdG (internal standard) were separated on a HILIC column with a mobile phase of 10. mM ammonium acetate: acetonitrile (1:9, v/v) within 10. min and detected by using a positive electrospray ionization interface under the selected reaction monitoring mode. The detection limits of 8-OHdG (corresponding to a signal-to-noise ratio of 3) for the HILIC-MS/MS system and the conventional method using a reversed-phase column with MS/MS were 1.0 and 26.0. fmol/injection, respectively. The proposed method makes it possible to monitor the basal level of urinary 8-OHdG from non-exposed healthy subjects and can be used for large-scale human studies. © 2012 Elsevier B.V. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.elsevier.com/locate/issn/15700232 | |||||
