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Retinoid X receptor α in human liver is regulated by miR-34a
https://doi.org/10.24517/00015094
https://doi.org/10.24517/00015094cd7f8cef-b3a4-4a71-9d72-cd4d89cb099a
| 名前 / ファイル | ライセンス | アクション |
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PH-PR-NAKAJIMA-M-179.pdf (367.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2017-10-04 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Retinoid X receptor α in human liver is regulated by miR-34a | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24517/00015094 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
Oda, Yuki
× Oda, Yuki× Nakajima, Miki× Tsuneyama, Koichi× Takamiya, Masataka× Aoki, Yasuhiro× Fukami, Tatsuki× Yokoi, Tsuyoshi |
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| 著者別表示 |
中嶋, 美紀
× 中嶋, 美紀
× 深見, 達基
× 横井, 毅
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| 提供者所属 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | 金沢大学ナノ生命科学研究所 / 金沢大学医薬保健研究域薬学系 | |||||||||||
| 書誌情報 |
Biochemical Pharmacology 巻 90, 号 2, p. 179-187, 発行日 2014-07-15 |
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| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0006-2952 | |||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA00564486 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isVersionOf | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1016/j.bcp.2014.05.002 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | Elsevier | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Retinoid X receptor α (RXRα) forms a heterodimer with numerous nuclear receptors to regulate drug- or lipid-metabolizing enzymes. In this study, we investigated whether human RXRα is regulated by microRNAs. Two potential recognition elements of miR-34a were identified in the RXRα mRNA: one in the coding region and the other in the 3′-untranslated region (3′-UTR). Luciferase assays revealed that miR-34a recognizes the element in the coding region. The overexpression of miR-34a in HepG2 cells significantly decreased the endogenous RXRα protein and mRNA levels. The stability of RXRα mRNA was decreased by the overexpression of miR-34a, indicating that miR-34a negatively regulates RXRα expression by facilitating mRNA degradation. We found that the miR-34a-dependent down-regulation of RXRα decreases the induction of CYP26 and the transactivity of CYP3A4. miR-34a has been reported to be up-regulated by p53, which has an ability to promote liver fibrosis. The p53 activation resulted in an increase of the miR-34a level and a decrease of the RXRα protein level. In addition, the miR-34a levels in eight fibrotic livers were higher than those in six normal livers, and the reverse trend was found for the RXRα protein levels. An inverse correlation was observed between the miR-34a and the RXRα protein levels in the 14 samples. Taken together, the data show that miR-34a negatively regulates RXRα expression in human liver, and affects the expression of its downstream genes. This miR-34a-dependent regulation might be the underlying mechanism responsible for the decreased expression of the RXRα protein in fibrotic livers. © 2014 Elsevier Inc. | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | AM | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||
| 関連URI | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | http://www.elsevier.com/locate/issn/00062952 | |||||||||||
