| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-04 |
| タイトル |
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|
タイトル |
Regulation of Cytochrome b5 Expression by miR-223 in Human Liver: Effects on Cytochrome P450 Activities |
| 言語 |
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言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00015109 |
|
ID登録タイプ |
JaLC |
| 著者 |
Takahashi, Kei
Oda, Yuki
Toyoda, Yasuyuki
Fukami, Tatsuki
Yokoi, Tsuyoshi
Nakajima, Miki
|
| 著者別表示 |
深見, 達基
横井, 毅
中嶋, 美紀
|
| 提供者所属 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
金沢大学ナノ生命科学研究所 / 金沢大学医薬保健研究域薬学系 |
| 書誌情報 |
Pharmaceutical Research
巻 31,
号 3,
p. 780-794,
発行日 2014-03-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0724-8741 |
| NCID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10632083 |
| DOI |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1007/s11095-013-1200-7 |
| 出版者 |
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出版者 |
Springer |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Purpose: Cytochrome b 5 (b 5) is a hemoprotein that transfers electrons to several enzymes to fulfill functions in fatty acid desaturation, methemoglobin reduction, steroidogenesis, and drug metabolism. Despite the importance of b 5, the regulation of b 5 expression in human liver remains largely unknown. We investigated whether microRNA (miRNA) might be involved in the regulation of human b 5. Methods: Twenty-four human liver specimens were used for correlation analysis. In silico analysis and luciferase assay were performed to determine whether the predicted miRNAs functionally target to b 5. The miR-223 was overexpressed into HepG2 cells infected with adenovirus expressing human cytochrome P450. Results: In human livers, the b 5 protein levels were not positively correlated with the b 5 mRNA levels, and miR-223 levels were inversely correlated with the b 5 mRNA levels or the translational efficiencies. The luciferase assay showed that miR-223 functionally binds to the element in the 3′-untranslated region of b 5 mRNA. The overexpression of miR-223 significantly reduced the endogenous b 5 protein level and the mRNA stability in HepG2 cells. Moreover, the overexpression of miR-223 significantly reduced CYP3A4-catalyzed testosterone 6β-hydroxylation activity and CYP2E1-catalyzed chlorzoxazone 6-hydroxylase activity but not CYP1A2-catalyzed 7-ethoxyresorufin O-deethylase activity. Conclusions: miR-223 down-regulates b 5 expression in the human liver, modulating P450 activities. © 2013 Springer Science+Business Media New York. |
| 著者版フラグ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
PH-PR-NAKAJIMA-M-780.pdf (617.5 kB)
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