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子宮内膜症性卵巣嚢胞から分離した子宮内膜症上皮不死化細胞株の樹立
http://hdl.handle.net/2297/35533
http://hdl.handle.net/2297/3553378e31a56-ed96-4a8f-8bbf-09a2836e6b47
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00044397-122-2-37-38.pdf (476.4 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-10-04 | |||||
| タイトル | ||||||
| タイトル | 子宮内膜症性卵巣嚢胞から分離した子宮内膜症上皮不死化細胞株の樹立 | |||||
| タイトル | ||||||
| タイトル | Creation of immortalised epithelial cells from ovarian endometrioma | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | epithelial cells | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | immortalisation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | oestrogen | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ovarian endometrioma | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | progestin | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
保野, 由紀子
× 保野, 由紀子 |
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| 書誌情報 |
金沢大学十全医学会雑誌 = Journal of the Juzen Medical Society 巻 122, 号 2, p. 37-38, 発行日 2013-06-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0022-7226 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00044397 | |||||
| 出版者 | ||||||
| 出版者 | 金沢大学十全医学会 = The Juzen Medical Society Kanazawa University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Epithelial cells of endometriotic tissues are difficult to propagate in vitro as experimental material is scarce owing to their limited life span. However, there is an increasing concern regarding their malignant transformation in ovaries. The present study sought to generate their stable culture system.Methods and Results :Purified epithelial cells isolated from ovarian endometriomas using microscopic manipulation were successfully immortalised by combinatorial transfection of human cyclinD1, cdk4 and human telomerase reverse transcriptase (hTERT) genes, whereas the introduction of hTERT alone, or together with cdk4, was insufficient for immortalisation, leading to cellular senescence. We confirmed stable cytokeratin expression in the immortalised cells, proving their epithelial origin. These cells expressed progesterone receptor B and showed significant growth inhibition by various progestins. Oestrogen receptor (ER) expression was detected in these cells, albeit at low levels. Additional overexpression of ERα generated stable cells with oestrogen-dependent growth activation. Soft-agar colony formation assay and nude mice xenograft experiments demonstrated that these cells, even those with additional inactivation of p53, did not have transformed phenotypes. Conclusion: We for the first time generated immortalised epithelial cells from ovarian endometrioma that retained sex steroid responsiveness. These cells are invaluable tools not only for the consistent in vitro work but also for the study of molecular pathogenesis or carcinogenesis of endometriosis. © 2012 Cancer Research UK All rights reserved. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | [総説 / Reviews][博士課程優秀論文] 第11回 高安賞優秀論文賞受賞論文, British Journal of Cancer 106(6), pp.1205-1213(2012年2月掲載), Bono, Y., Kyo, S., Takakura, M., Maida, Y., Mizumoto, Y., Nakamura, M., Nomura, K., Kiyono, T., Inoue, M. "Creation of immortalised epithelial cells from ovarian endometrioma.", http://dx.doi.org/10.1038/bjc.2012.26 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
