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Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Genenase I
https://doi.org/10.24517/00027375
https://doi.org/10.24517/0002737558f166c5-f23d-4ef5-a56f-64c73f8244c4
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
CA-PR-MATSUMOTO-K-478.pdf (1.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2017-10-05 | |||||||
| タイトル | ||||||||
| タイトル | Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Genenase I | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00027375 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Hayata, Daichika
× Hayata, Daichika× Fukuta, Kazuhiro× 松本, 邦夫× Adachi, Eri× Hanada, Keigo× Adachi, Kiichi× Nakamura, Toshikazu |
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| 著者別表示 |
松本, 邦夫
× 松本, 邦夫
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| 提供者所属 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 金沢大学がん研究所附属分子標的がん医療研究開発センター | |||||||
| 書誌情報 |
Journal of Biotechnology 巻 133, 号 4, p. 478-485, 発行日 2008-02-29 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0168-1656 | |||||||
| NCID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA10458361 | |||||||
| DOI | ||||||||
| 関連タイプ | isVersionOf | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1016/j.jbiotec.2007.11.006 | |||||||
| 出版者 | ||||||||
| 出版者 | Elsevier | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Hepatocyte growth factor (HGF) is biosynthesized as a biologically inactive, single-chain form (pro-HGF). Its activation is associated with cleavage at Arg494-Val495 into a two-chain mature form composed of disulfide-linked α- and β-chains. Because serum is a major source of HGF activator (the predominant serine protease responsible for the processing of pro-HGF), serum-free production of recombinant, two-chain HGF had not been established. In this study, to enable serum-free production of two-chain HGF, we generated engineered human pro-HGFs that can be specifically cleaved and activated by Genenase I. Since Genenase I specifically cleaves the C-terminus of the His-Tyr sequence, which does not exist in human HGF, Arg494 (the C-terminus of the HGF α-chain) was replaced by His-Tyr, Ala-Ala-His-Tyr, Pro-Gly-His-Tyr, or Pro-Gly-Ala-Ala-His-Tyr. Genenase I cleaved engineered pro-HGFs specifically at the replaced amino acid sequences, forming a disulfide-linked two-chain form. The cleavage was most efficient in the case of the Pro-Gly-Ala-Ala-His-Tyr sequence, and cleaved HGFs displayed biological activities identical to those of wild-type HGF. Considering a potential medical application of HGF, the present technique is valuable because it enables the production of recombinant, two-chain HGF entirely without serum and extends the choice of host cells and organisms for recombinant production. © 2007 Elsevier B.V. All rights reserved. | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
