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Static and dynamic hypergravity responses of osteoblasts and osteoclasts in medaka scales
http://hdl.handle.net/2297/34731
http://hdl.handle.net/2297/34731899ff812-6818-4e9d-ab06-0d16f35f1d4a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
NA-PR-YANO-SUZUMKI-217.pdf (728.7 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-05 | |||||
| タイトル | ||||||
| タイトル | Static and dynamic hypergravity responses of osteoblasts and osteoclasts in medaka scales | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Yano, Sachiko
× Yano, Sachiko× Kitamura, Kei-ichiro× Satoh, Yusuke× Nakano, Masaki× Hattori, Atsuhiko× Sekiguchi, Toshio× Ikegame, Mika× Nakashima, Hiroshi× Omori, Katsunori× Hayakawa, Kazuichi× Chiba, Atsuhiko× Sasayama, Yuichi× Ejiri, Sadakazu× Mikuni-Takagaki, Yuko× Mishima, Hiroyuki× Funahashi, Hisayuki× Sakamoto, Tatsuya× Suzuki, Nobuo |
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| 書誌情報 |
Zoological Science 巻 30, 号 3, p. 217-223, 発行日 2013-03-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0289-0003 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10545874 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.2108/zsj.30.217 | |||||
| 出版者 | ||||||
| 出版者 | Zoological Society of Japan = 日本動物学会 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Fish scales are a form of calcified tissue similar to that found in human bone. In medaka scales, we detected both osteoblasts and osteoclasts and subsequently developed a new scale assay system. Using this system, we analyzed the osteoblastic and osteoclastic responses under 2-, 3-, and 4-gravity (G) loading by both centrifugation and vibration. After loading for 10 min, the scales from centrifugal and vibration loading were incubated for 6 and 24 hrs, respectively, after which the osteoblastic and osteoclastic activities were measured. Osteoblastic activity significantly increased under 2- to 4-G loading by both centrifugation and vibration. In contrast, we found that osteoclastic activity significantly decreased under 2- and 3-G loading in response to both centrifugation and vibration. Under 4-G loading, osteoclastic activity also decreased on centrifugation, but significantly increased under 4-G loading by vibration, concomitant with markedly increased osteoblastic activity. Expression of the receptor activator of the NF-αB ligand (RANKL), an activation factor of osteoclasts expressed in osteoblasts, increased significantly under 4-G loading by vibration but was unchanged by centrifugal loading. A protein sequence similar to osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, was found in medaka using our sequence analysis. The ratio of RANKL/OPG-like mRNAs in the vibration-loaded scales was significantly higher than that in the control scales, although there was no difference between centrifugal loaded scales and the control scales. Accordingly, medaka scales provide a useful model by which to analyze bone metabolism in response to physical strain. © 2013 Zoological Society of Japan. | |||||
| 権利 | ||||||
| 権利情報 | © 2013 Zoological Society of Japan | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.zoology.or.jp/ | |||||
