| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-12-25 |
| タイトル |
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タイトル |
Folding of the Cerebral Cortex Requires Cdk5 in Upper-Layer Neurons in Gyrencephalic Mammals |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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|
ID登録 |
10.24517/00049603 |
|
ID登録タイプ |
JaLC |
| 著者 |
Shinmyo, Yohei
Terashita, Yukari
Duong, Tung Anh Dinh
Horiike, Toshihide
Kawasumi, Muneo
Hosomichi, Kazuyoshi
Tajima, Atsushi
Kawasaki, Hiroshi
|
| 著者別表示 |
新明, 洋平
堀池, 俊秀
細道, 一善
田嶋, 敦
河﨑, 洋志
|
| 提供者所属 |
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内容記述タイプ |
Other |
|
内容記述 |
金沢大学医薬保健研究域医学系 |
| 書誌情報 |
Cell Reports
巻 20,
号 9,
p. 2131-2143,
発行日 2017-08-29
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2211-1247 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.celrep.2017.08.024 |
| 出版者 |
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出版者 |
Elsevier B.V. |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Folds in the cerebral cortex in mammals are believed to be key structures for accommodating increased cortical neurons in the cranial cavity. However, the mechanisms underlying cortical folding remain largely unknown, mainly because genetic manipulations for the gyrencephalic brain have been unavailable. By combining in utero electroporation and the CRISPR/Cas9 system, we succeeded in efficient gene knockout of Cdk5, which is mutated in some patients with classical lissencephaly, in the gyrencephalic brains of ferrets. We show that Cdk5 knockout in the ferret cerebral cortex markedly impaired cortical folding. Furthermore, the results obtained from the introduction of dominant-negative Cdk5 into specific cortical layers suggest that Cdk5 function in upper-layer neurons is more important for cortical folding than that in lower-layer neurons. Cdk5 inhibition induced severe migration defects in cortical neurons. Taken together, our findings suggest that the appropriate positioning of upper-layer neurons is critical for cortical folding. © 2017 The Author(s) |
| 権利 |
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|
権利情報 |
Copyright © 2017 The Author(s) |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連URI |
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識別子タイプ |
URI |
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関連識別子 |
http://www.elsevier.com/locate/issn/2211-1247 |
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|
関連名称 |
http://www.elsevier.com/locate/issn/2211-1247 |
ME-PR-KAWASAKI-H-2131.pdf (6.5 MB)
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