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Simple and cost-effective assay for isolating invasive living cells
https://doi.org/10.24517/00053869
https://doi.org/10.24517/00053869a5a6c50e-a8dd-40fc-9cce-2ef8ff9ccd7b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
GS-PR-TAKINO-T-0036.pdf (1.8 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2019-04-12 | |||||||
| タイトル | ||||||||
| タイトル | Simple and cost-effective assay for isolating invasive living cells | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00053869 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Takino, Takahisa
× Takino, Takahisa× Sato, Hiroshi× Seiki, Motoharu |
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| 著者別表示 |
滝野, 隆久
× 滝野, 隆久
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| 提供者所属 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 金沢大学国際基幹教育院GS教育系 / Institute of Liberal Arts and Science | |||||||
| 書誌情報 |
BioTechniques 巻 65, 号 3, p. 137-142, 発行日 2018-09 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0736-6205 | |||||||
| item_4_source_id_11 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA1066297X | |||||||
| DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.2144/btn-2018-0036 | |||||||
| 出版者 | ||||||||
| 出版者 | Future Medicine | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | A modified invasion assay using a three-dimensional collagen gel was developed that enables isolation of invasive living cells; it was named the invading cell trapping (iCT) assay. A small cell strainer consisting of a nylon mesh with 40-μm2 pores was used, and collagen gel layers formed across the membrane. Test cells were seeded in the lower gel layer and invasive cells were attracted upward and trapped in the upper gel. After incubation, the collagen gel layers in cell strainers were easily separated and living cells in the gel were counted and analyzed. An advantage of the iCT assay is that it can capture living invasive cells in the upper gel while leaving noninvasive ones in the lower layer. Further enrichment of the two cell populations can be achieved by repeating the assay. Thus, the iCT assay allows comparative analysis of invasive versus noninvasive cells. | |||||||
| 権利 | ||||||||
| 権利情報 | copyright © | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連URI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.future-science.com/doi/10.2144/btn-2018-0036 | |||||||
| 関連名称 | https://www.future-science.com/doi/10.2144/btn-2018-0036 | |||||||
