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HPV-16 impairs the subcellular distribution and levels of expression of protein phosphatase 1γ in cervical malignancy
https://doi.org/10.24517/00062680
https://doi.org/10.24517/00062680b08b1651-171d-4c15-a3da-b47eae21e12e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ME-PR-MAEDA-D-230.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2021-06-28 | |||||||
| タイトル | ||||||||
| タイトル | HPV-16 impairs the subcellular distribution and levels of expression of protein phosphatase 1γ in cervical malignancy | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| ID登録 | ||||||||
| ID登録 | 10.24517/00062680 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
Seiki, Takayuki
× Seiki, Takayuki× Nagasaka, Kazunori× Kranjec, Christian× Kawana, Kei× Maeda, Daichi× Nakamura, Hiroe× Taguchi, Ayumi× Matsumoto, Yoko× Arimoto, Takahide× Wada-Hiraike, Osamu× Oda, Katsutoshi× Nakagawa, Shunsuke× Yano, Tetsu× Fukayama, Masashi× Banks, Lawrence× Osuga, Yutaka× Fujii, Tomoyuki |
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| 著者別表示 |
前田, 大地
× 前田, 大地
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| 提供者所属 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 金沢大学医薬保健研究域医学系 | |||||||
| 書誌情報 |
BMC Cancer 巻 15, 号 1, p. 230, 発行日 2015-04-07 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1471-2407 | |||||||
| NCID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA12034763 | |||||||
| DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1186/s12885-015-1141-0 | |||||||
| 出版者 | ||||||||
| 出版者 | BioMed Central Ltd. | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Background: The high risk Human Papillomavirus (HPV) E6 oncoproteins play an essential role in the development of cervical malignancy. Important cellular targets of E6 include p53 and the PDZ domain containing substrates such as hScrib and Dlg. We recently showed that hScrib activity was mediated in part through recruitment of protein phosphatase 1γ (PP1γ). Methods: Expression patterns of hScrib and PP1γ were assessed by immunohistochemistry of HPV-16 positive cervical intraepithelial neoplasm (CIN), classified as CIN1 (n=4), CIN2 (n=8), CIN3 (n=8), cervical carcinoma tissues (n=11), and HPV-negative cervical tissues (n=8), as well as by subfractionation assay of the HPV-16 positive cervical cancer cell lines, CaSki and SiHa. To explore the effects of the HPV-16 oncoproteins, we have performed siRNA knockdown of E6/E7 expression, and monitored the effects on the expression patterns of hScrib and PP1γ. Results: We show that PP1γ levels in HPV-16 positive tumour cells are reduced in an E6/E7 dependent manner. Residual PP1γ in these cells is found mostly in the cytoplasm as opposed to the nucleus where it is predominantly found in normal cells. We have found a striking concordance with redistribution in the pattern of expression (9/11; 81.8%) and loss of PP1γ expression in HPV-16 positive cervical tumours (2/11; 18.2%). Furthermore, this loss of PP1γ expression and redistribution in the pattern of expression occurs progressively as the lesions develop (8/8; 100%). Conclusion: Together, these results suggest that PP1γ may be a novel target of the HPV-16 oncoproteins and indicate that it might be a potential novel biomarker for HPV-16 induced malignancy. © Seiki et al. | |||||||
| 権利 | ||||||||
| 権利情報 | Copyright © BioMed Central Ltd. | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | http://www.biomedcentral.com/bmccancer/ | |||||||
| 関連名称 | http://www.biomedcentral.com/bmccancer/ | |||||||
| 関連URI | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://bmccancer.biomedcentral.com/articles/10.1186/s12885-015-1141-0 | |||||||
| 関連名称 | https://bmccancer.biomedcentral.com/articles/10.1186/s12885-015-1141-0 | |||||||
