{"_buckets": {"deposit": "31a6f720-c6cd-4fd7-a969-dcf713ec15c5"}, "_deposit": {"created_by": 18, "id": "57771", "owners": [18], "pid": {"revision_id": 0, "type": "depid", "value": "57771"}, "status": "published"}, "_oai": {"id": "oai:kanazawa-u.repo.nii.ac.jp:00057771", "sets": ["2834"]}, "author_link": ["22117", "100987"], "item_9_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2001-10-22", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "2p.", "bibliographicVolumeNumber": "1998 – 1999", "bibliographic_titles": [{"bibliographic_title": "平成11(1999)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要"}, {"bibliographic_title": "1999 Fiscal Year Final Research Report Summary", "bibliographic_titleLang": "en"}]}]}, "item_9_creator_33": {"attribute_name": "著者別表示", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ohta, Kazuhide"}], "nameIdentifiers": [{"nameIdentifier": "22117", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20283129", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=20283129"}, {"nameIdentifier": "20283129", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000020283129"}]}]}, "item_9_description_21": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "EBVコード化小RNA1(EBV encoded small RNA1,EBER1)は、あらゆるEBV感染細胞に全ての感染様式において大量に発現しているRNAであるが、その発現調節機構は明らかではない。しかし現在、EBER1を標的とした生体内局所ハイブリダイゼーション(in situ hybridization,ISH)が、EBV関連疾患の診断に広く利用されている。今回の検討では、EBER1を標的としたISHにフローサイトメトリー(flow cytometry,FCM)による解析を組み合わせた解析法(ISH/FCM)によりEBV感染細胞の定量的評価を試みた。その結果、フルオレッセン標識EBER1プローブを用いたISH/FCMによって、従来のアルカリフォスファターゼ標識EBER1プローブによるISHに比べ、より定量的な評価が可能であった。しかし、EBER1陽性細胞1%以下の、感染細胞比率の低い検体での定量的評価は現時点では困難であった。また今回の検討ではEBER1発現の程度は種々の細胞株によって異なり、また同一細胞株においても蛍光輝度の分布が幅広く、個々の細胞におけるEBER1発現量が異なることが明かとなった。このことより、EBER1発現は、宿主細胞種の違いや、個々の細胞により異なる因子によって調節されていることが示唆された。本法を用いた臨床検体の解析では、対照及び伝染性単核症では末梢血中にEBER1陽性細胞は検出されず、HLHやCAEBVでは容易に検出できた。したがってEBER1 ISH/FCMによりHLHやCAEBVにおける感染細胞や感染様式のモニターが比較的容易にできると考えられた。また本法は、他のEBV関連遺伝子や感染細胞の表面抗原との多因子解析により、さらに簡便に多くの情報をもたらすと考えられた。", "subitem_description_type": "Abstract"}, {"subitem_description": "Epstein-Barr virus (EBV) is prevalent among adult population and it persists within B lymphocytes in latent forms throughout life. Infection. mononucleosis is a well recognized, self-limited EBV- related acute infection. However, increasing numbers of illness are now known to be associated with this particular herpes virus, such as hemophagocytic lymphohistiocytosis (HLH), chronic active EBV infection (CAEBV) and various malignant neoplasms, including nasopharyngeal carcinoma, Hodgikin\u0027s disease and gastric cartinoma. It has been suggested by studying these diseases that the cellular targets of vital infection and the modes of the latency of viruses greatly the modify pathogenesis of EBV-related illnesses. EBV-encoded small RNA-1 (EBER1) is expressed in abundance in virtually every cell infected with EBV, regardless of the mode of latency and therefore its expression is often used as a sensitive indicator of EBV association with certain illnesses. In this study, the clinical relevance of in situ hybridization flowctyometric analysis (ISH/FCM) of EBER1 expression was evaluated to identify EBV-infected cells quantitatively. Preliminary experiments showed that ISH/FCM using a fluorescence-labeled probe for EBER1 mRNA enabled more quantitative evaluation of the fraction of the infected cells within a sample population than standard ISH using alkaline phosphatase-labeled probe. However, the former was less sensitive when the fraction of the infected cells was smaller than 1 percent. Levels of EBER1 expression were various among different cell lines and the distribution of the fluorescence intensity within a single cell population is relatively wide, indicating that EBER1 expression is controlled by multiple mechanisms. Importantly, the virus-infected cells were easily detectable within peripheral lymphocytes from patients with HLH and CAEBV, but not from normal controls or patients with acute infectious mononucleosis. EBER1 ISH/FCM may serve as a useful tool to monitor sequentially the target cells and the mode of infection in these patients with relative ease. Further information will be available by applying multiparameter analysis of EBER1 expression in combination with different EBV-related gene expression and surface antigen analysis.", "subitem_description_type": "Abstract"}]}, "item_9_description_22": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "研究課題/領域番号:10670709, 研究期間(年度):1998 – 1999", "subitem_description_type": "Other"}, {"subitem_description": "出典：「EBウイルス感染症の多様性と病態発現機序に関する研究;標的細胞特異的病態発現におけるウイルス遺伝子発現調節とクローン成立機構」研究成果報告書　課題番号10670709\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所））\n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-10670709/106707091999kenkyu_seika_hokoku_gaiyo/）を加工して作成", "subitem_description_type": "Other"}]}, "item_9_description_5": {"attribute_name": "提供者所属", "attribute_value_mlt": [{"subitem_description": "金沢大学医学部・附属病院", "subitem_description_type": "Other"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24517/00064041", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_relation_28": {"attribute_name": "関連URI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://kaken.nii.ac.jp/ja/search/?kw=20283129"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/ja/search/?kw=20283129", "subitem_relation_type_select": "URI"}}, {"subitem_relation_name": [{"subitem_relation_name_text": "https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-10670709/"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-10670709/", "subitem_relation_type_select": "URI"}}, {"subitem_relation_name": [{"subitem_relation_name_text": "https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-10670709/106707091999kenkyu_seika_hokoku_gaiyo/"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-10670709/106707091999kenkyu_seika_hokoku_gaiyo/", "subitem_relation_type_select": "URI"}}]}, "item_9_version_type_25": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "太田, 和秀"}], "nameIdentifiers": [{"nameIdentifier": "100987", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20283129", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=20283129"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-09-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ME-PR-OHTA-K-kaken 2001-2p.pdf", "filesize": [{"value": "110.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 110900.0, "url": {"label": "ME-PR-OHTA-K-kaken 2001-2p.pdf", "url": "https://kanazawa-u.repo.nii.ac.jp/record/57771/files/ME-PR-OHTA-K-kaken 2001-2p.pdf"}, "version_id": "a6a54a3c-3bab-486c-bd21-86c7a42eaa76"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "EB virus (EBV)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PCR method", "subitem_subject_scheme": "Other"}, {"subitem_subject": "EBV terminal repeat", "subitem_subject_scheme": "Other"}, {"subitem_subject": "EBER-1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "in situ hybridization", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "EBウイルス感染症の多様性と病態発現機序に関する研究;標的細胞特異的病態発現におけるウイルス遺伝子発現調節とクローン成立機構", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "EBウイルス感染症の多様性と病態発現機序に関する研究;標的細胞特異的病態発現におけるウイルス遺伝子発現調節とクローン成立機構"}, {"subitem_title": "Pathogenesis of Diverse Clinical Manifestations of EBV-Related Disorders : Mechanisms of Target-Cell Specific Infection, Regulation of Viral Gene Expression and Clonal Expansion.", "subitem_title_language": "en"}]}, "item_type_id": "9", "owner": "18", "path": ["2834"], "permalink_uri": "https://doi.org/10.24517/00064041", "pubdate": {"attribute_name": "公開日", "attribute_value": "2021-09-06"}, "publish_date": "2021-09-06", "publish_status": "0", "recid": "57771", "relation": {}, "relation_version_is_last": true, "title": ["EBウイルス感染症の多様性と病態発現機序に関する研究;標的細胞特異的病態発現におけるウイルス遺伝子発現調節とクローン成立機構"], "weko_shared_id": -1}