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In vivo differences between two optical isomers of radioiodinated o-iodo-transdecalinvesamicol for Use as a radioligand for the vesicular acetylcholine transporter
https://doi.org/10.24517/00065239
https://doi.org/10.24517/00065239ae3453f8-2eec-41bf-8c21-c8abcac4b001
| 名前 / ファイル | ライセンス | アクション |
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ME-PR-SHIBA-K-0146719.pdf (297.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| 公開日 | 2022-01-28 | |||||||||||||||||
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| タイトル | In vivo differences between two optical isomers of radioiodinated o-iodo-transdecalinvesamicol for Use as a radioligand for the vesicular acetylcholine transporter | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| ID登録 | ||||||||||||||||||
| ID登録 | 10.24517/00065239 | |||||||||||||||||
| ID登録タイプ | JaLC | |||||||||||||||||
| 著者 |
Uno, Izumi
× Uno, Izumi× Kozaka, Takashi× Miwa, Daisuke× Kitamura, Yoji× Azim, Mohammad Anwar-ul× Ogawa, Kazuma× Taki, Junichi× Kinuya, Seigo× Shiba, Kazuhiro |
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| 著者別表示 |
小阪, 孝史
× 小阪, 孝史
× 北村, 暘二
× 小川, 数馬
× 瀧, 淳一
× 絹谷, 清剛
× 柴, 和弘
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| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | 金沢大学疾患モデル総合研究センター | |||||||||||||||||
| 書誌情報 |
PLoS ONE 巻 11, 号 1, p. e0146719, 発行日 2016-01-11 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 1932-6203 | |||||||||||||||||
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| 関連タイプ | isIdenticalTo | |||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | 10.1371/journal.pone.0146719 | |||||||||||||||||
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| 出版者 | Public Library of Science | |||||||||||||||||
| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Purpose To develop a superior VAChT imaging probe for SPECT, radiolabeled (-)-OIDV and (+)-OIDV were isolated and investigated for differences in their binding affinity and selectivity to VAChT, as well as their in vivo activities. Procedures Radioiodinated o-iodo-trans-decalinvesamicol ([125I]OIDV) has a high binding affinity for vesicular acetylcholine transporter (VAChT) both in vitro and in vivo. Racemic [125I]OIDV was separated into its two optical isomers (-)-[125I]OIDV and (+)-[125I]OIDV by HPLC. To investigate VAChT binding affinity (Ki) of two OIDV isomers, in vitro binding assays were performed. In vivo biodistribution study of each [125I]OIDV isomer in blood, brain regions and major organs of rats was performed at 2,30 and 60 min post-injection. In vivo blocking study were performed to reveal the binding selectivity of two [125I]OIDV isomers to VAChT in vivo. Ex vivo autoradiography were performed to reveal the regional brain distribution of two [125I]OIDV isomers and (-)-[123I]OIDV for SPECT at 60 min postinjection. Results VAChT binding affinity (Ki) of (-)-[125I]OIDV and (+)-[125I]OIDV was 22.1 nM and 79.0 nM, respectively. At 2 min post-injection, accumulation of (-)-[125I]OIDV was the same as that of (+)-[125I]OIDV. However, (+)-[125I]OIDV clearance from the brain was faster than (-)-[125I]OIDV. At 30 min post-injection, accumulation of (-)-[125I]OIDV (0.62 ± 0.10%ID/g) was higher than (+)-[125I]OIDV (0.46 ± 0.07%ID/g) in the cortex. Inhibition of OIDV binding showed that (-)-[125I]OIDV was selectively accumulated in regions known to express VAChT in the rat brain, and ex vivo autoradiography further confirmed these results showing similar accumulation of (-)-[125I]OIDV in these regions. Furthermore, (-)-[123I]OIDV for SPECT showed the same regional brain distribution as (-)-[125I]OIDV. Conclusion These results suggest that radioiodinated (-)-OIDV may be a potentially useful tool for studying presynaptic cholinergic neurons in the brain. |
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| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | CC-BY 4.0 | |||||||||||||||||
| 権利 | ||||||||||||||||||
| 権利情報 | Copyright © 2016 Uno et al.This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited., Published by the Public Library of Science | |||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
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| 識別子タイプ | URI | |||||||||||||||||
| 関連識別子 | http://www.plosone.org/ | |||||||||||||||||
| 関連名称 | http://www.plosone.org/ | |||||||||||||||||
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| 関連識別子 | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0146719 | |||||||||||||||||
| 関連名称 | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0146719 | |||||||||||||||||
