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Development and evaluation of a novel 99mTc-labeled annexin A5 for early detection of response to chemotherapy
https://doi.org/10.24517/00065241
https://doi.org/10.24517/000652417ff23dce-5bab-4e1f-bb63-11814e81c6f9
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ME-PR-SHIBA-K-e81191.pdf (444.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2022-01-31 | |||||
| タイトル | ||||||
| タイトル | Development and evaluation of a novel 99mTc-labeled annexin A5 for early detection of response to chemotherapy | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| ID登録 | ||||||
| ID登録 | 10.24517/00065241 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
Ogawa, Kazuma
× Ogawa, Kazuma× Ohtsuki, Katsuichi× Shibata, Tomomi× Aoki, Miho× Nakayama, Morio× Kitamura, Yoji× Ono, Masahiro× Ueda, Masashi× Doue, Tomoki× Onoguchi, Masahisa× Shiba, Kazuhiro× Odani, Akira |
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| 著者別名 |
小川, 数馬
× 小川, 数馬× 北村, 暘二× 小野口, 昌久× 柴, 和弘× 小谷, 明 |
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| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学疾患モデル総合研究センター | |||||
| 書誌情報 |
PLoS ONE 巻 8, 号 12, p. e81191, 発行日 2013-12-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1932-6203 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1371/journal.pone.0081191 | |||||
| 出版者 | ||||||
| 出版者 | Public Library of Science | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | 99mTc-HYNIC-annexin A5 can be considered as a benchmark in the field of apoptosis imaging. However, 99mTc-HYNIC-annexin A5 has characteristics of high uptake and long retention in non-target tissues such as kidney and liver. To minimize this problem, we developed a novel 99mTc-labeled annexin A5 using a bis(hydroxamamide) derivative [C3(BHam)2] as a bifunctional chelating agent, and evaluated its usefulness as an imaging agent for detecting apoptosis. The amino group of C3(BHam)2 was converted to a maleimide group, and was coupled to thiol groups of annexin A5 pretreated with 2-iminothiolane. 99mTc labeling was performed by a ligand exchange reaction with 99mTc-glucoheptonate. Biodistribution experiments for both 99mTc-C3(BHam)2-annexin A5 and 99mTc-HYNIC-annexin A5 were performed in normal mice. In addition, in tumor-bearing mice, the relationship between the therapeutic effects of chemotherapy (5-FU) and the tumor accumulation of 99mTc-C 3(BHam)2-annexin A5 just after the first treatment of 5-FU was evaluated. 99mTc-C3(BHam)2-annexin A5 was prepared with a radiochemical purity of over 95%. In biodistribution experiments, 99mTc-C3(BHam)2-annexin A5 had a much lower kidney accumulation of radioactivity than 99mTc-HYNIC- annexin A5. In the organs for metabolism, such as liver and kidney, radioactivity after the injection of 99mTc-HYNIC-annexin A5 was residual for a long time. On the other hand, radioactivity after the injection of 99mTc-C3(BHam)2-annexin A5 gradually decreased. In therapeutic experiments, tumor growth in the mice treated with 5-FU was significantly inhibited. Accumulation of 99mTc-C 3(BHam)2-annexin A5 in tumors significantly increased after 5-FU treatment. The accumulation of radioactivity in tumor correlated positively with the counts of TUNEL-positive cells. These findings suggest that 99mTc-C3(BHam)2-annexin A5 may contribute to the efficient detection of apoptotic tumor response after chemotherapy. © 2013 Ogawa et al. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | CC-BY 4.0 | |||||
| 権利 | ||||||
| 権利情報 | Copyright © 2013 Ogawa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.plosone.org/ | |||||
| 関連名称 | http://www.plosone.org/ | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081191 | |||||
| 関連名称 | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081191 | |||||
