| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-03 |
| タイトル |
|
|
タイトル |
Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00010416 |
|
ID登録タイプ |
JaLC |
| その他のタイトル |
|
|
値 |
セルロース表面におけるセルラーゼの交通渋滞が加水分解効率を低下させる |
| 著者 |
Igarashi, Kiyohiko
Uchihashi, Takayuki
Koivula, Anu
Wada, Masahisa
Kimura, Satoshi
Okamoto, Tetsuaki
Penttilä, Merja
Ando, Toshio
Samejima, Masahiro
|
| 著者別表示 |
五十嵐, 圭日子
内橋, 貴之
鮫島, 正浩
|
| 提供者所属 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
金沢大学ナノ生命科学研究所 / 金沢大学理工研究域数物科学系 |
| 書誌情報 |
Science
巻 333,
号 6047,
p. 1279-1282,
発行日 2011-09-02
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0036-8075 |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00835277 |
| DOI |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1126/science.1208386 |
| 出版者 |
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出版者 |
American Association for the Advancement of Science |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Abstract A deeper mechanistic understanding of the saccharification of cellulosic biomass could enhance the efficiency of biofuels development. We report here the real-time visualization of crystalline cellulose degradation by individual cellulase enzymes using an advanced version of high-speed atomic force microscopy. Trichoderma reesei 2 cellobiohydrolase I (TrCel7A) molecules were observed to slide unidirectionally along the crystalline cellulose surface, but at one point exhibited collective halting analogous to a traffic jam. Changing the crystalline polymorphic form of cellulose by an ammonia treatment increased the apparent number of accessible lanes on the crystalline surface and consequently the number of moving cellulase molecules. Treatment of this bulky crystalline cellulose simultaneously or separately with T. reesei cellobiohydrolase II (TrCel6A) resulted in a significant increase in the proportion of mobile enzyme molecules on the surface. Cellulose was completely degraded by the synergistic action between the two enzymes. |
| 権利 |
|
|
権利情報 |
© 2011 American Association for the Advancement of Science. |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連URI |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://www.sciencemag.org/content/333/6047/1279.full |
SC-PR-UCHIHASHI-T-1279.pdf (4.4 MB)
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