| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-12-05 |
| タイトル |
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タイトル |
Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.24517/00029380 |
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ID登録タイプ |
JaLC |
| 著者 |
Kase, Yoichi
Ikari, Takahiro
Sekiguchi, Toshio
Sato, Masayuki
Ogiso, Shouzo
Kawada, Tsuyoshi
Matsubara, Shin
Satake, Honoo
Sasayama, Yuichi
Endo, Masato
Kitamura, Kei-ichiro
Hattori, Atsuhiko
Watanabe, Takushi X.
Maruyama, Yusuke
Watanabe, Yoshinari
Funahashi, Hisayuki
Kambegawa, Akira
Suzuki, Nobuo
|
| 著者別表示 |
関口, 俊男
佐藤, 政行
笹山, 雄一
北村, 敬一郎
渡辺, 良成
鈴木, 信雄
|
| 書誌情報 |
Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
巻 211,
p. 77-83,
発行日 2017-09-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1095-6433 |
| NCID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11183804 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.cbpa.2017.06.007 |
| 出版者 |
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出版者 |
Elsevier |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The nucleotide sequence of a sardine preprocalcitonin precursor has been determined from their ultimobranchial glands in the present study. From our analysis of this sequence, we found that sardine procalcitonin was composed of procalcitonin amino-terminal cleavage peptide (N-proCT) (53 amino acids), CT (32 amino acids), and procalcitonin carboxyl-terminal cleavage peptide (C-proCT) (18 amino acids). As compared with C-proCT, N-proCT has been highly conserved among teleosts, reptiles, and birds, which suggests that N-proCT has some bioactivities. Therefore, both sardine N-proCT and sardine CT were synthesized, and their bioactivities for osteoblasts and osteoclasts were examined using our assay system with goldfish scales that consisted of osteoblasts and osteoclasts. As a result, sardine N-proCT (10− 7 M) activated osteoblastic marker enzyme activity, while sardine CT did not change. On the other hand, sardine CT (10− 9 to 10− 7 M) suppressed osteoclastic marker enzyme activity, although sardine N-proCT did not influence enzyme activity. Furthermore, the mRNA expressions of osteoblastic markers such as type 1 collagen and osteocalcin were also promoted by sardine N-proCT (10− 7 M) treatment; however, sardine CT did not influence their expressions. The osteoblastic effects of N-proCT lack agreement. In the present study, we can evaluate exactly the action for osteoblasts because our scale assay system is very sensitive and it is a co-culture system for osteoblasts and osteoclasts with calcified bone matrix. Both CT and N-proCT seem to influence osteoblasts and osteoclasts and promote bone formation by different actions in teleosts. © 2017 Elsevier Inc. |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Embargo Period 12 months |
| 権利 |
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権利情報 |
Copyright © Elsevier (CC-BY NC ND) |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連URI |
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識別子タイプ |
URI |
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関連識別子 |
http://www.elsevier.com/locate/issn/10956433 |
OC-PR-SUZUKI-N-77.pdf (765.9 kB)
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