| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2018-03-01 |
| タイトル |
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タイトル |
A novel method for measuring human hepatic lipase activity in postheparin plasma |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.24517/00050262 |
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ID登録タイプ |
JaLC |
| 著者 |
Imamura, S.
Kobayashi, Junji
Sakasegawa, S.
Nohara, Atsushi
Nakajima, Kenichi
Kawashiri, Masa-aki
Inazu, Akihiro
Yamagishi, Masakazu
Koizumi, Junji
Mabuchi, Hiroshi
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| 著者別表示 |
小林, 淳二
野原, 淳
中嶋, 憲一
川尻, 剛照
稲津, 明広
山岸, 正和
小泉, 順二
馬渕, 宏
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| 提供者所属 |
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内容記述タイプ |
Other |
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内容記述 |
金沢大学医薬保健研究域医学系 |
| 書誌情報 |
Journal of Lipid Research
巻 48,
号 2,
p. 453-457,
発行日 2007-02
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0022-2275 |
| NCID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00701215 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1194/jlr.D600022-JLR200 |
| 出版者 |
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出版者 |
American Society for Biochemistry and Molecular Biology |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The objective of this study was to establish a hepatic lipase (HL) assay method that can be applied to automatic clinical analyzers. Seventy-four hyperlipidemic subjects (men/women 45/29) were recruited. Lipase activity was assayed measuring the increase in absorbance at 546 nm due to quinonediimine dye production. Reaction mixture R-1 contained 50 mM Tris-HCl (pH 9.5), 0.5 mM glycerol-1,2-dioleate, 0.4% (unless otherwise noted) polyoxyethylenenonylphenylether, 3 mM ATP, 3 mM MgCl2, 1.5 mM CaCl2, monoacylglycerol-specific lipase, glycerol kinase, glycerol-3-phosphate oxidase, 0.075% N,N-bis-(4-sulfobutyl)-3-methylaniline-2 Na, peroxidase, ascorbic acid oxidase. Reaction mixture R-2 contained 50 mM Tris-HCl (pH9.5), 0.15% 4-aminoantypirine. Automated assay for activity was performed with a Model 7080 Hitachi analyzer. In the lipase assay, 160 μl of R-1 was incubated at 37°C with 3 μl of samples for 5 min, and 80 μl of R-2 was added. Within-run coefficient of variations was 0.9-1.0%. Calibration curve of lipase activity was linear (r = 0.999) between 0 and 320 U/l. Analytical recoveries of purified HL added to plasma were 96.6-99.8%. HL activity in postheparin plasma measured in this method had a closer correlation with HL mass by a sandwich ELISA (r = 0.888, P , 0.0001) than those in the conventional method using [ 14C-]triolein (r = 0.730, P < 0.0001). This assay method for HL activity can be applied to an automatic clinical analyzer. Copyright © 2007 by the American Society for Biochemistry and Molecular Biology, Inc. |
| 権利 |
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権利情報 |
Copyright © American Society for Biochemistry and Molecular Biology |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連URI |
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識別子タイプ |
URI |
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関連識別子 |
http://www.jlr.org/content/48/2/453 |
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関連名称 |
http://www.jlr.org/content/48/2/453 |
ME-PR-YAMAGISHI-M-453.pdf (205.5 kB)
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