{"_buckets": {"deposit": "03ed8347-004b-4e13-87a1-834fe2d06677"}, "_deposit": {"created_by": 18, "id": "43920", "owners": [18], "pid": {"revision_id": 0, "type": "depid", "value": "43920"}, "status": "published"}, "_oai": {"id": "oai:kanazawa-u.repo.nii.ac.jp:00043920", "sets": ["1134"]}, "author_link": ["72634", "72531", "72536", "70168", "72534", "68650", "72537", "72538", "70167", "72533", "72577", "72532", "72530", "69602", "72633", "72535", "72539", "69361"], "item_4_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "457", "bibliographicPageStart": "453", "bibliographicVolumeNumber": "48", "bibliographic_titles": [{"bibliographic_title": "Journal of Lipid Research"}]}]}, "item_4_creator_33": {"attribute_name": "著者別表示", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "小林, 淳二"}], "nameIdentifiers": [{"nameIdentifier": "72532", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "野原, 淳"}], "nameIdentifiers": [{"nameIdentifier": "72533", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "中嶋, 憲一"}], "nameIdentifiers": [{"nameIdentifier": "72534", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "川尻, 剛照"}], "nameIdentifiers": [{"nameIdentifier": "72535", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90345637", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90345637"}]}, {"creatorNames": [{"creatorName": "稲津, 明広"}], "nameIdentifiers": [{"nameIdentifier": "72536", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80293348", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80293348"}]}, {"creatorNames": [{"creatorName": "山岸, 正和"}], "nameIdentifiers": [{"nameIdentifier": "72537", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70393238", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70393238"}]}, {"creatorNames": [{"creatorName": "小泉, 順二"}], "nameIdentifiers": [{"nameIdentifier": "72538", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20161846", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=20161846"}]}, {"creatorNames": [{"creatorName": "馬渕, 宏"}], "nameIdentifiers": [{"nameIdentifier": "72539", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "00019960", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=00019960"}]}]}, "item_4_description_21": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The objective of this study was to establish a hepatic lipase (HL) assay method that can be applied to automatic clinical analyzers. Seventy-four hyperlipidemic subjects (men/women 45/29) were recruited. Lipase activity was assayed measuring the increase in absorbance at 546 nm due to quinonediimine dye production. Reaction mixture R-1 contained 50 mM Tris-HCl (pH 9.5), 0.5 mM glycerol-1,2-dioleate, 0.4% (unless otherwise noted) polyoxyethylenenonylphenylether, 3 mM ATP, 3 mM MgCl2, 1.5 mM CaCl2, monoacylglycerol-specific lipase, glycerol kinase, glycerol-3-phosphate oxidase, 0.075% N,N-bis-(4-sulfobutyl)-3-methylaniline-2 Na, peroxidase, ascorbic acid oxidase. Reaction mixture R-2 contained 50 mM Tris-HCl (pH9.5), 0.15% 4-aminoantypirine. Automated assay for activity was performed with a Model 7080 Hitachi analyzer. In the lipase assay, 160 μl of R-1 was incubated at 37°C with 3 μl of samples for 5 min, and 80 μl of R-2 was added. Within-run coefficient of variations was 0.9-1.0%. Calibration curve of lipase activity was linear (r = 0.999) between 0 and 320 U/l. Analytical recoveries of purified HL added to plasma were 96.6-99.8%. HL activity in postheparin plasma measured in this method had a closer correlation with HL mass by a sandwich ELISA (r = 0.888, P , 0.0001) than those in the conventional method using [ 14C-]triolein (r = 0.730, P \u003c 0.0001). This assay method for HL activity can be applied to an automatic clinical analyzer. Copyright © 2007 by the American Society for Biochemistry and Molecular Biology, Inc.", "subitem_description_type": "Abstract"}]}, "item_4_description_5": {"attribute_name": "提供者所属", "attribute_value_mlt": [{"subitem_description": "金沢大学医薬保健研究域医学系", "subitem_description_type": "Other"}]}, "item_4_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24517/00050262", "subitem_identifier_reg_type": "JaLC"}]}, "item_4_publisher_17": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Society for Biochemistry and Molecular Biology "}]}, "item_4_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1194/jlr.D600022-JLR200", "subitem_relation_type_select": "DOI"}}]}, "item_4_relation_28": {"attribute_name": "関連URI", "attribute_value_mlt": [{"subitem_relation_name": 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