Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2018-04-13 |
タイトル |
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タイトル |
The CD45+ fraction in murine adipose tissue derived stromal cells harbors immune-inhibitory inflammatory cells |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
ID登録 |
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ID登録 |
10.24517/00050487 |
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ID登録タイプ |
JaLC |
著者 |
Nasti, Alessandro
Sakai, Yoshio
Seki, Akihiro
Buffa, Geraldine Belen
Komura, Takuya
Mochida, Hatsune
Yamato, Masatoshi
Yoshida, Keiko
Ho, Tuyen T. B.
Takamura, Masayuki
Usui, Soichiro
Wada, Takashi
Honda, Masao
Kaneko, Shuichi
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著者別表示 |
酒井, 佳夫
餅田, 初音
吉田, 佳子
高村, 雅之
薄井, 荘一郎
和田, 隆志
本多, 政夫
金子, 周一
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提供者所属 |
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内容記述タイプ |
Other |
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内容記述 |
金沢大学医薬保健研究域医学系 |
書誌情報 |
European Journal of Immunology
巻 47,
号 12,
p. 2163-2174,
発行日 2017-12
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ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0014-2980 |
NCID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00639610 |
DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1002/eji.201646835 |
出版者 |
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出版者 |
Wiley |
抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Stromal cells in adipose tissue are useful for repair/regenerative therapy as they harbor a substantial number of mesenchymal stem cells; therefore, freshly isolated autologous uncultured adipose tissue derived stromal cells (u-ADSCs) are useful for regenerative therapy, and obviate the need for mesenchymal stem cells. We evaluated the therapeutic effect of murine u-ADSCs and sorted subsets of u-ADSCs in a concanavalin A (ConA) induced murine model of hepatitis, as well as their characteristics. We found that 10–20% of u-ADSCs expressed the CD45 leukocyte-related antigen. CD68, which is a marker of macrophages (MΦs), was expressed by 50% of CD45+ u-ADSCs. About 90% of CD68+CD45+ cells expressed CD206 antigen, which is a marker of inhibitory M2-type MΦs. Genes related to M2-type MUs were especially more highly expressed by CD45+CD206+ u-ADSCs than by CD45− u-ADSCs. CD45+ u-ADSCs inhibited the expression of cytokines/chemokines and suppressed the proliferation of splenocytes stimulated with ConA. We observed that not only whole u-ADSCs, but also the CD45+ subset of u-ADSCs ameliorated the ConA-induced hepatitis in mice. In conclusion, we show that freshly isolated murine u-ADSCs were effective against acute hepatitis, and CD45+ u-ADSCs acting phenotypically and functionally like M2-type MΦs, contributed to the repair of liver tissue undergoing inflammation. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Embargo Period 12 months |
権利 |
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権利情報 |
Copyright © Wiley |
著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |