| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-05-12 |
| タイトル |
|
|
タイトル |
Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00066042 |
|
ID登録タイプ |
JaLC |
| 著者 |
Sakai, Katsuya
Passioura, Toby
Sato, Hiroki
Ito, Kenichiro
Furuhashi, Hiroki
Umitsu, Masataka
Takagi, Junichi
Kato, Yukinari
Mukai, Hidefumi
Warashina, Shota
Zouda, Maki
Watanabe, Yasuyoshi
Yano, Seiji
Shibata, Mikihiro
Suga, Hiroaki
Matsumoto, Kunio
|
| 著者別表示 |
酒井, 克也
佐藤, 拓輝
矢野, 聖二
柴田, 幹大
松本, 邦夫
|
| 提供者所属 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
金沢大学がん進展制御研究所 |
| 書誌情報 |
Nature Chemical Biology
巻 15,
号 6,
p. 598-606,
発行日 2019-05-17
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1552-4450 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1552-4469 |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11994319 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1038/s41589-019-0285-7 |
| 出版者 |
|
|
出版者 |
Nature Research / Springer Nature |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Activation of hepatocyte growth factor (HGF) by proteolytic processing is triggered in cancer microenvironments, and subsequent signaling through the MET receptor is involved in cancer progression. However, the structure of HGF remains elusive, and few small/medium-sized molecules can modulate HGF. Here, we identified HiP-8, a macrocyclic peptide consisting of 12 amino acids, which selectively recognizes active HGF. Biochemical analysis and real-time single-molecule imaging by high-speed atomic force microscopy demonstrated that HiP-8 restricted the dynamic domains of HGF into static closed conformations, resulting in allosteric inhibition. Positron emission tomography using HiP-8 as a radiotracer enabled noninvasive visualization and simultaneous inhibition of HGF–MET activation status in tumors in a mouse model. Our results illustrate the conformational change in proteolytic activation of HGF and its detection and inhibition by a macrocyclic peptide, which may be useful for diagnosis and treatment of cancers. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Embargo Period 6 months |
| 権利 |
|
|
権利情報 |
Copyright © The Author(s), under exclusive licence to Springer Nature America, Inc. 2019 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連URI |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.nature.com/articles/s41589-019-0285-7 |
|
|
関連名称 |
https://www.nature.com/articles/s41589-019-0285-7 |
| 関連URI |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.nature.com/nchembio/ |
|
|
関連名称 |
https://www.nature.com/nchembio/ |
| 関連URI |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.nature.com/ |
|
|
関連名称 |
https://www.nature.com/ |
CA-PR-SAKAI-K-598.pdf (2.1 MB)
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